Gao Dong, Lin Jiu-Mao, Zheng Liang-Pu
Fujian College of Traditional Chinese Medicine, Fuzhou.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2007 Jun;27(6):527-30.
To investigate the effect of Xuefu Zhuyu Decoction (XFZYD) on the number, phenotype, cell cycle and colony formation of bone marrow hematopoietic stem cells (HSC) in mice.
Kunming mice were randomly divided into 4 groups: the control group, the low- (3.25 g/kg), middle- (6.5 g/kg) and high-dose (13.0 g/kg) XFZYD groups. After they were medicated by gastrogavage respectively with saline or corresponding dose of XFZYD for 7 days, their bone marrow HSC were separated and counted. The phenotype Sca and cell cycle of HSC were detected by flow cytometer, and the colony formation was determined with semisolid methyl media culture.
No obvious difference in the number of mononuclear cell, suspended cell and colony production was found among all the groups (P > 0.05); while the expression of CD34 and Sca-1 increased in the low-dose XFZYD group, but in the middle-dose XFZYD group increase only showed in Sca-1 expression.
XFZYD plays a role of removing blood stasis and promoting regeneration through improving hematopoietic function by means of increasing the number and enhancing the function of premature HSC.
探讨血府逐瘀汤对小鼠骨髓造血干细胞（HSC）数量、表型、细胞周期及集落形成的影响。
将昆明小鼠随机分为4组：对照组、血府逐瘀汤低剂量（3.25 g/kg）组、中剂量（6.5 g/kg）组和高剂量（13.0 g/kg）组。分别用生理盐水或相应剂量的血府逐瘀汤灌胃给药7天后，分离并计数其骨髓造血干细胞。采用流式细胞仪检测造血干细胞的表型Sca及细胞周期，用半固体甲基纤维素培养基培养法测定集落形成情况。
各组间单个核细胞、悬浮细胞数量及集落生成数比较，差异无统计学意义（P＞0.05）；低剂量血府逐瘀汤组CD34及Sca-1表达增加，中剂量血府逐瘀汤组仅Sca-1表达增加。
血府逐瘀汤可通过增加早期造血干细胞数量、增强其功能来改善造血功能，发挥活血化瘀、促进再生的作用。
