Huang Dan, Li Shun Xiang, Cai Guang Xian, Yue Chun Hua, Wei Li Jun, Zhang Ping
Provincial Key Laboratory of Drug Research, Hunan Academy of Traditional Chinese Medicine, Changsha, PR China.
Biol Pharm Bull. 2008 Feb;31(2):312-5. doi: 10.1248/bpb.31.312.
In present paper, the properties of molecular authentication combined with the fingerprints of high performance liquid chromatography (HPLC) were validated by analyzing ten batches of Fructus Evodiae samples (the dried nearly ripe fruit of Evodia rutaecarpa (JUSS.) BENTH., Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG or Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG). The results of this investigation show that the similarities of internal transcribed spacer (ITS) sequences were almost 100% in Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG, 97% in Evodia rutaecarpa (JUSS.) BENTH., and 96% in Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG. The percentage of identity between the two groups of Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG and Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG is almost 96%, but the identity among the group of these three species is only 73%. The results show that Fructus Evodiae comes from three species respectively. The fingerprints of HPLC show that Fructus Evodiae revealed 20 major common peaks. And the three species have almost the same chemical constituents. ITS sequence fingerprint combining the fingerprint of HPLC can not only be developed to identify and distinguish the three species in detail, but also can be used for optimizing location where Fructus Evodiae has much higher bioactive constituents and yield.
本文通过对10批吴茱萸样品（吴茱萸、石虎或疏毛吴茱萸干燥近成熟果实）进行分析，验证了分子鉴定与高效液相色谱（HPLC）指纹图谱相结合的特性。研究结果表明，疏毛吴茱萸的内转录间隔区（ITS）序列相似度几乎为100%，吴茱萸为97%，石虎为96%。疏毛吴茱萸和石虎两组之间的一致性百分比几乎为96%，但这三个物种组之间的一致性仅为73%。结果表明，吴茱萸分别来自三个物种。HPLC指纹图谱显示，吴茱萸有20个主要共有峰。并且这三个物种的化学成分几乎相同。ITS序列指纹图谱结合HPLC指纹图谱不仅可以用于详细鉴别和区分这三个物种，还可以用于优化吴茱萸生物活性成分含量和产量更高的产地。
