Utp8p是一种核仁tRNA结合蛋白，在酿酒酵母中与核tRNA输出机制的组分形成复合物。
Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae.
作者信息
Strub Benjamin R, Eswara Manoja B K, Pierce Jacqueline B, Mangroo Dev
机构信息
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
出版信息
Mol Biol Cell. 2007 Oct;18(10):3845-59. doi: 10.1091/mbc.e06-11-1016. Epub 2007 Jul 18.
Abstract
Utp8p is an essential nucleolar component of the nuclear tRNA export machinery in Saccharomyces cerevisiae. It is thought to act at a step between tRNA maturation/aminoacylation and translocation of the tRNA across the nuclear pore complex. To understand the function of Utp8p in nuclear tRNA export, a comprehensive affinity purification analysis was conducted to identify proteins that interact with Utp8p in vivo. In addition to finding proteins that have been shown previously to copurify with Utp8p, a number of new interactions were identified. These interactions include aminoacyl-tRNA synthetases, the RanGTPase Gsp1p, and nuclear tRNA export receptors such as Los1p and Msn5p. Characterization of the interaction of Utp8p with a subset of the newly identified proteins suggests that Utp8p most likely transfer tRNAs to the nuclear tRNA export receptors by using a channeling mechanism.
摘要
Utp8p是酿酒酵母中核tRNA输出机制的一种必需核仁成分。它被认为在tRNA成熟/氨酰化与tRNA穿过核孔复合体的转运之间的某个步骤发挥作用。为了了解Utp8p在核tRNA输出中的功能，进行了全面的亲和纯化分析，以鉴定在体内与Utp8p相互作用的蛋白质。除了发现先前已证明与Utp8p共纯化的蛋白质外，还鉴定了许多新的相互作用。这些相互作用包括氨酰-tRNA合成酶、RanGTP酶Gsp1p以及核tRNA输出受体，如Los1p和Msn5p。对Utp8p与一部分新鉴定蛋白质相互作用的表征表明，Utp8p很可能通过一种通道机制将tRNA转移到核tRNA输出受体。
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