Russo J, Soule H D, McGrath C, Rich M A
J Natl Cancer Inst. 1976 Feb;56(2):279-82. doi: 10.1093/jnci/56.2.279.
A stable cell line (MCF-7), derived from a pleural effusion of a patient with metastatic breast carcinoma, was maintained in these laboratories for more than 3 years in conventional monolayer culture. To further characterize the tumor origin of the MCF-7 line, cells were grown on collagen-coated cellulos sponges. On the three-dimensional sponge matrix, the cells formed clusters, ductlike structures, and lumina similar to the patterns observed in the antecedent primary tumor and in the pleural metastasis. The similarity between the original tumor and the cells grown in sponge suggested that the MCF-7 cells did in fact retain the potential to express the histologic patterns of tumor, even in the absence of stroma support. This study confirmed the utility of sponge culture for the investigation of the retention of tumor characteristics by cultured cells of neoplastic origin.
一种源自转移性乳腺癌患者胸腔积液的稳定细胞系（MCF-7），在这些实验室中采用传统单层培养法维持了3年多。为了进一步明确MCF-7细胞系的肿瘤起源特征，将细胞接种于胶原包被的纤维素海绵上。在三维海绵基质上，细胞形成了细胞簇、导管样结构和管腔，类似于在先前的原发性肿瘤和胸膜转移灶中观察到的模式。原始肿瘤与在海绵上生长的细胞之间的相似性表明，即使在没有基质支持的情况下，MCF-7细胞实际上仍保留表达肿瘤组织学模式的潜力。本研究证实了海绵培养在研究肿瘤源性培养细胞保留肿瘤特征方面的实用性。
