[甘肃省文县人群中的无症状利什曼原虫感染]
[Asymptomatic Leishmania infection in human population of Wenxian County, Gansu Province].
作者信息
Wang Jun-Yun, Feng Yu, Gao Chun-Hua, Jin Chang-Fa, Chen Sheng-Bang, Zhang Chou-Ji, He Jin-Ping, Yang Chen-Ming, Yang Yue-Tao, Bao Yi-Fang
机构信息
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007 Feb 28;25(1):62-4.
OBJECTIVE
To analyze the status of Leishmania infantum asymptomatic infection in human population of a Kala-azar endemic area in Wenxian County, Gansu Province, and to evaluate the tests used.
METHODS
Blood samples were tested by PCR using two pairs of primers, RV1-RV2 and K13A-K13B, for detecting Leishmania-specific DNA. ELISA and rK39-dipstick were used to detect Leishmania-specific antibodies.
RESULTS
The positive rate of PCR, ELISA and rK39-dipstick was 30.9%(83/269). 24.2%(65/269) and 0 (0/269) respectively.
CONCLUSION
The prevalence of asymptomatic infection of L. infantum in humans is high in the area. PCR test based on RV1-RV2 and K13A-K13B primer pairs is a sensitive and specific method for detecting the asymptomatic infection.
目的
分析甘肃省文县黑热病流行区人群中婴儿利什曼原虫无症状感染状况，并对所采用的检测方法进行评价。
方法
采用RV1-RV2和K13A-K13B两对引物，通过聚合酶链反应（PCR）检测血液样本中的利什曼原虫特异性DNA。采用酶联免疫吸附试验（ELISA）和rK39免疫试纸条检测利什曼原虫特异性抗体。
结果
PCR、ELISA和rK39免疫试纸条的阳性率分别为30.9%（83/269）、24.2%（65/269）和0（0/269）。
结论
该地区人群中婴儿利什曼原虫无症状感染率较高。基于RV1-RV2和K13A-K13B引物对的PCR检测是检测无症状感染的一种灵敏且特异的方法。
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