对经紫外线照射的单纯疱疹病毒转化的细胞中存在的病毒DNA进行定量分析。
Quantitation of the viral DNA present in cells transformed by UV-irradiated herpes simplex virus.
作者信息
Davis D B, Kingsbury D T
出版信息
J Virol. 1976 Mar;17(3):788-93. doi: 10.1128/JVI.17.3.788-793.1976.
Abstract
Two cell lines biochemically transformed by UV-irradiated herpes simplex virus (HSV) each contain virus DNA. A comparison of the kinetics of reassociation of 3H-labeled HSV DNA in the presence and absence of either clone 139 (HSV-1 transformed) or clone 207 (HSV-2 transformed) DNA showed that the presence of transformed cell DNA increased the rate of reassociation of approximately 10% of the viral genome while having no effect on the remaining 90%. The Cot1/2 of this reaction was approximately 1,000 in each cell type, as compared to approximately 3,000 for the cellular unique sequences. These results suggest the presence of four to six copies of a 10% fragment of the virus DNA per cell. The DNA from a hamster fibroblast cell line morphologically transformed by UV-irradiated HSV-2 (333-8-9) did not affect the rate of reassociation of HSV-2 DNA, indicating that these cells had less than 3% of a viral genome present.
摘要
两个经紫外线照射的单纯疱疹病毒（HSV）进行生化转化的细胞系均含有病毒DNA。对在存在或不存在克隆139（HSV - 1转化）或克隆207（HSV - 2转化）DNA的情况下，3H标记的HSV DNA重新结合动力学的比较表明，转化细胞DNA的存在使约10%的病毒基因组的重新结合速率增加，而对其余90%没有影响。与细胞独特序列的约3000相比，每种细胞类型中该反应的Cot1/2约为1000。这些结果表明每个细胞中存在病毒DNA 10%片段的四到六个拷贝。来自经紫外线照射的HSV - 2（333 - 8 - 9）形态转化的仓鼠成纤维细胞系的DNA不影响HSV - 2 DNA的重新结合速率，表明这些细胞中存在的病毒基因组不到3%。
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