Frenkel N, Locker H, Cox B, Roizman B, Rapp F
J Virol. 1976 Jun;18(3):885-93. doi: 10.1128/JVI.18.3.885-893.1976.
Analyses of the hybridization kinetics of labeled herpes simplex virus 2 (HSV-2) DNA with DNA from five hamster cell lines transformed by UV light-irradiated HSV-2 revealed the following. (i) Viral DNA sequences were detected in all five cell lines tested. (ii) None of the cell lines contained the full complement of HSV-2 DNA. (iii) The amount of viral DNA present in the cells varied in different transformed cell lines and ranged from 8 to 32% of the HSV-2 DNA genome in 1 to 3 copies/cell. (iv) Two parallel passages of the same cell line (333-2-29) differed in the amount of viral DNA they contained. We also compared the viral DNA sequences present in (i) one transformed cell line (333-8-9) propagated serially in culture for 80 passages, (ii) a tumor produced by inoculation of a newborn hamster with the 333-8-9 cells, and (iii) a cell line derived from a hamster tumor as above and propagated in culture for 32 passages. The results show that viral DNA present in the hamster tumor and in the cells derived from the tumor had a lower sequence complexity than that present in the original serially passaged 333-8-9 cell line.
对标记的单纯疱疹病毒2（HSV - 2）DNA与经紫外线照射的HSV - 2转化的五种仓鼠细胞系的DNA进行杂交动力学分析，结果如下：（i）在所有测试的五种细胞系中均检测到病毒DNA序列。（ii）没有一个细胞系包含HSV - 2 DNA的完整互补序列。（iii）细胞中存在的病毒DNA量在不同的转化细胞系中有所不同，范围为每个细胞1至3个拷贝，占HSV - 2 DNA基因组的8%至32%。（iv）同一细胞系（333 - 2 - 29）的两次平行传代所含病毒DNA量不同。我们还比较了以下情况中存在的病毒DNA序列：（i）在培养中连续传代80次的一个转化细胞系（333 - 8 - 9）；（ii）用333 - 8 - 9细胞接种新生仓鼠产生的肿瘤；（iii）如上所述从仓鼠肿瘤衍生并在培养中传代32次的细胞系。结果表明，仓鼠肿瘤以及肿瘤衍生细胞中存在的病毒DNA的序列复杂性低于原始连续传代的333 - 8 - 9细胞系中存在的病毒DNA。
