Mahadevan-Jansen A, Mitchell M F, Ramanujam N, Utzinger U, Richards-Kortum R
Biomedical Engineering Program, University of Texas, Austin, USA.
Photochem Photobiol. 1998 Sep;68(3):427-31.
The goal of this study was to develop a compact fiber optic probe to measure near infrared Raman spectra of human cervical tissue in vivo for the clinical diagnosis of cervical precancers. A Raman spectrometer and fiber optic probe were designed, constructed and tested. The probe was first tested using standards with known Raman spectra, and then the probe was used to acquire Raman spectra from normal and precancerous cervical tissue in vivo. Raman spectra of cervical tissue could be acquired in vivo in 90 s using incident powers comparable to the threshold limit values for laser exposure of the skin. Although some silica signal obscured tissue Raman bands below 900 cm-1, Raman features from cervical tissue could clearly be discerned with an acceptable signal-to-noise ratio above 900 cm-1. The success of the Raman probe described here indicates that near infrared Raman spectra can be measured in vivo from cervical tissues. Increasing the power of the excitation source could reduce the integration time to below 20 s.
本研究的目的是开发一种紧凑型光纤探头，用于在体内测量人宫颈组织的近红外拉曼光谱，以用于宫颈癌前病变的临床诊断。设计、构建并测试了一台拉曼光谱仪和光纤探头。该探头首先使用具有已知拉曼光谱的标准品进行测试，然后用于在体内获取正常和癌前宫颈组织的拉曼光谱。使用与皮肤激光照射阈值限值相当的入射功率，可在90秒内于体内获取宫颈组织的拉曼光谱。尽管在900 cm-1以下一些二氧化硅信号掩盖了组织拉曼谱带，但在900 cm-1以上，宫颈组织的拉曼特征能够以可接受的信噪比清晰辨别。此处所述拉曼探头的成功表明，可以在体内测量宫颈组织的近红外拉曼光谱。增加激发源的功率可将积分时间缩短至20秒以下。
