Naito S, Min Y-K, Sugata K, Osanai O, Kitahara T, Hiruma H, Hamaguchi H
Tochigi Research Laboratories, Kao Corporation, Tochigi, Japan.
Skin Res Technol. 2008 Feb;14(1):18-25. doi: 10.1111/j.1600-0846.2007.00255.x.
BACKGROUND/AIMS: Although chemical information on the dermis in vivo is highly important in skin research, an efficient method for gathering this information is yet to be developed. Here, we demonstrate that newly developed near-infrared (1064 nm) excited Raman spectroscopy is a powerful method for chemical analysis of human skin in vivo.
We used a laboratory-constructed Raman spectrometer equipped with a highly sensitive near-infrared detector (Hamamatsu Photonics), an optical fiber probe and a 1064 nm Nd:YAG laser. Raman spectra of porcine skin (in vitro) and human skin (in vivo) were measured with this spectrometer.
The Raman spectrum of porcine skin measured from the outer side resembles that of the dermis more than that of the epidermis. The Raman spectra of human skin (cheek, forehead, inner forearm, outer forearm, palm) depend on the portion measured with the probe. The spectra of the forehead and inner forearm show larger lipid signals than that of the palm.
The Raman spectrum of skin measured with the 1064 nm Raman system primarily reflects the chemical composition of the dermis. The 1064 nm excited Raman spectroscopy is useful for research of the dermis and skin appendages.
背景/目的：尽管真皮在体内的化学信息在皮肤研究中非常重要，但尚未开发出一种有效的方法来收集这些信息。在此，我们证明新开发的近红外（1064nm）激发拉曼光谱是一种用于体内人体皮肤化学分析的强大方法。
我们使用了一台实验室构建的拉曼光谱仪，配备高灵敏度近红外探测器（滨松光子学）、光纤探头和1064nm Nd:YAG激光。用该光谱仪测量猪皮肤（体外）和人体皮肤（体内）的拉曼光谱。
从外侧测量的猪皮肤拉曼光谱与真皮的拉曼光谱更相似，而不是表皮的。人体皮肤（脸颊、额头、前臂内侧、前臂外侧、手掌）的拉曼光谱取决于用探头测量的部位。额头和前臂内侧的光谱显示出比手掌更大的脂质信号。
用1064nm拉曼系统测量的皮肤拉曼光谱主要反映真皮的化学成分。1064nm激发拉曼光谱可用于真皮和皮肤附属器的研究。
