Michaeli D, Martin G R, Kettman J, Benjamini E, Leung D Y, Blatt B A
Laboratory of Medical Entomology, Kaiser Foundation Research Institute, San Francisco, California, USA.
Science. 1969 Dec 19;166(3912):1522-4. doi: 10.1126/science.166.3912.1522.
After being injected with collagen from rat or guinea pig skin, rabbits form high titer, species-specific antibodies to collagen. Antibody is directed primarily against the alpha2 chain of collagen with little reaction with the alpha1 chain. The amino terminal peptide of cyanogen bromide cleavage of the alpha2 chain of guinea pig skin collagen, alpha2-CBl, effectively inhibited the antigen-antibody reaction, an indication that a major antigenic determinant of collagen is located at the amino terminal of the alpha2 chain.
给兔子注射大鼠或豚鼠皮肤的胶原蛋白后，兔子会形成针对胶原蛋白的高滴度、种属特异性抗体。抗体主要针对胶原蛋白的α2链，与α1链反应较弱。豚鼠皮肤胶原蛋白α2链经溴化氰裂解产生的氨基末端肽α2-CBl能有效抑制抗原-抗体反应，这表明胶原蛋白的一个主要抗原决定簇位于α2链的氨基末端。
