Yu Jian-sheng, Yu Jian-ping
Tongren Vocational Institute, Tongren 554300, China.
Zhong Yao Cai. 2007 Aug;30(8):1008-12.
A method for separation and purification of total alkaloids from Macleaya cordata with macroporous resin was studied, with the content and recovery rate of total alkaloids as indexes. The results were as follows: The static adsorption capacity of AB-8 type resin was 104. 65 mg/g, the static elution ratio were 95.9% , the dynamic adsorption capacity of AB-8 type resin was 96.5 mg/g, the recovery rate was more than 91.24% and the purity was more than 90%. AB-8 type resin was the best for separating and purificating Macleaya cordata in total alkaloids. The optimum conditions is: the eluant is 90% alcohol and 2-3 times as the volume of the resin, the volume of the resin is 10.4 times of total alkaloids in sample, concentration of total alkaloids of sample is 21.57 mg/ml and current velocity of 2-3 ml/min, pH value of sample is 7-8.
以大孔树脂为分离材料，以总生物碱的含量和回收率为指标，对博落回总生物碱的分离纯化方法进行了研究。结果表明：AB-8型树脂静态吸附量为104.65mg/g，静态洗脱率为95.9%，动态吸附量为96.5mg/g，回收率大于91.24%，纯度大于90%。AB-8型树脂是分离纯化博落回总生物碱的最佳树脂。最佳工艺条件为：洗脱剂为90%乙醇，用量为树脂体积的23倍；树脂用量为样品中总生物碱体积的10.4倍；样品总生物碱浓度为21.57mg/ml；流速为23ml/min；样品pH值为7~8。
