Qi Hua, Wang Yao, Bao Yan, Bassham Diane C, Chen Liang, Chen Qin-Fang, Hou Suiwen, Hwang Inhwan, Huang Li, Lai Zhibing, Li Faqiang, Liu Yule, Qiu Rongliang, Wang Hao, Wang Pengwei, Xie Qingjun, Zeng Yonglun, Zhuang Xiaohong, Gao Caiji, Jiang Liwen, Xiao Shi
Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, 510642, China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Adv Biotechnol (Singap). 2023 Oct 26;1(4):2. doi: 10.1007/s44307-023-00002-8.
In plants, autophagy is a conserved process by which intracellular materials, including damaged proteins, aggregates, and entire organelles, are trafficked to the vacuole for degradation, thus maintaining cellular homeostasis. The past few decades have seen extensive research into the core components of the central autophagy machinery and their physiological roles in plant growth and development as well as responses to biotic and abiotic stresses. Moreover, several methods have been established for monitoring autophagic activities in plants, and these have greatly facilitated plant autophagy research. However, some of the methodologies are prone to misuse or misinterpretation, sometimes casting doubt on the reliability of the conclusions being drawn about plant autophagy. Here, we summarize the methods that are widely used for monitoring plant autophagy at the physiological, microscopic, and biochemical levels, including discussions of their advantages and limitations, to provide a guide for studying this important process.
在植物中，自噬是一个保守的过程，通过该过程，包括受损蛋白质、聚集体和整个细胞器在内的细胞内物质被运输到液泡进行降解，从而维持细胞内稳态。在过去几十年里，人们对自噬核心机制的关键组分及其在植物生长发育以及对生物和非生物胁迫响应中的生理作用进行了广泛研究。此外，已经建立了几种监测植物自噬活性的方法，这些方法极大地促进了植物自噬研究。然而，其中一些方法容易被误用或误解，有时会让人对有关植物自噬得出的结论的可靠性产生怀疑。在此，我们总结了在生理、微观和生化水平上广泛用于监测植物自噬的方法，包括对其优缺点的讨论，以为研究这一重要过程提供指导。
