Zhang Xi, Xiao Hong-Bin, Xue Xing-Ya, Sun Yan-Guo, Liang Xin-Miao
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, PR China.
J Sep Sci. 2007 Aug;30(13):2059-69. doi: 10.1002/jssc.200700014.
A method was developed for the simultaneous identification of astragalosides (AGs) and isoflavonoids (IFs) in the roots of Astragalus membranaceus and Astragalus mongholicus by HPLC coupled with atmospheric pressure chemical ionization MS/MS (HPLC-APCI-MS/MS). Diagnostic fragment ions of AGs and different group of IFs were obtained with one AG and eight IF standards analyzed by CID-MS, which were adopted as characteristic MS/MS fingerprints for further identification of these compounds in the two Astragalus species by using HPLC-APCI-MS/MS. A total of 20 IFs and 10 AGs were identified or tentatively identified. Among them, six IFs were detected in A. membranaceus for the first time and five IFs were firstly identified in A. mongholicus. The results indicate that HPLC-APCI-MS/MS is a powerful tool for the simultaneous characterization of IFs and AGs in complex matrix.
建立了一种通过高效液相色谱-大气压化学电离串联质谱（HPLC-APCI-MS/MS）同时鉴定膜荚黄芪和蒙古黄芪根中黄芪皂苷（AGs）和异黄酮（IFs）的方法。通过CID-MS分析一种AG和八种IF标准品，获得了AGs和不同组IFs的诊断性碎片离子，将其作为特征性MS/MS指纹图谱，用于通过HPLC-APCI-MS/MS进一步鉴定这两种黄芪属植物中的这些化合物。共鉴定或初步鉴定出20种IFs和10种AGs。其中，6种IFs首次在膜荚黄芪中检测到，5种IFs首次在蒙古黄芪中鉴定出。结果表明，HPLC-APCI-MS/MS是在复杂基质中同时表征IFs和AGs的有力工具。
