Tang Li, Liu Ying, Wang Yeling, Long Chunlin
College of Life and Environmental Science, Minzu University of China, No. 27 Zhongguancun South Street, Beijing, People's Republic of China.
J Nat Med. 2010 Apr;64(2):182-6. doi: 10.1007/s11418-009-0381-1.
Radix astragali (Huangqi in Chinese) is a well-known traditional Chinese medicinal herb that has been used clinically in China for centuries to cure various diseases. To profile the antiviral constituents of this herb, a high-performance liquid chromatography-diode array detector-electrospray ionization-tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) analytical method was developed to separate and determinate the active part of the extract of Radix astragali, which showed potent inhibition of several viruses. By comparing their retention time and MS data with those obtained from the authentic compounds and the published data, a total of 18 compounds, comprising 11 flavonoids and 7 saponins, were identified. This study provides an approach to rapidly characterize bioactive constituents in traditional Chinese medicines (TCMs).
黄芪是一种著名的传统中草药，在中国临床上用于治疗各种疾病已有数百年历史。为了剖析这种草药的抗病毒成分，开发了一种高效液相色谱 - 二极管阵列检测器 - 电喷雾电离 - 串联质谱（HPLC-DAD-ESI-MS/MS）分析方法，以分离和测定黄芪提取物中具有强效抑制多种病毒作用的活性部分。通过将它们的保留时间和质谱数据与从 authentic 化合物获得的数据以及已发表的数据进行比较，共鉴定出 18 种化合物，包括 11 种黄酮类化合物和 7 种皂苷。本研究提供了一种快速表征中药生物活性成分的方法。
