尿 N-乙酰-β-D-氨基葡萄糖苷酶及其同工酶 A 和 B 在镀镉工人中的研究。

Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A & B in workers exposed to cadmium at cadmium plating.

机构信息

Regional Occupational Health Centre (Southern), Indian Council of Medical Research, Bangalore Medical College Campus, Bangalore-560 002, India.

出版信息

J Occup Med Toxicol. 2007 Jul 20;2:5. doi: 10.1186/1745-6673-2-5.

DOI:10.1186/1745-6673-2-5

PMID:17659077

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1971251/

Abstract

OBJECTIVE

The present study was carried out to determine the effect of cadmium exposure on Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B in workers exposed at cadmium plating.

METHODS

50 subjects using cadmium during cadmium plating formed the study group. An equal number of age-sex matched subjects working in administrative section formed the control group. Urinary cadmium levels were determined by using a flameless atomic absorption spectrophotometer. Urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B were determined by using spectrophotmetric method.

RESULTS

A significant increase of urinary total N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B profiles were noted in study as compared to controls. The levels of urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B profiles were positively and significantly correlated with cadmium levels in urine. Multiple regression analysis was used to assess the effect of urinary cadmium or life style confounding factors (age, BMI, smoking and alcohol consumption) on urinary N-acetyl-beta -D-glucosaminidase and its isoenzymes A and B. The analysis showed that the study subjects who had urine cadmium levels greater than 5 mug/g of creatinine, work duration >15 years, smoking and body mass index variables were significantly associated with urinary total N-acetyl-beta -D-glucosaminidase but not on isoenzymes A&B.

CONCLUSION

The results presented in this study shows that the increased levels of urinary N-acetyl-beta -D-glucosaminidase observed in cadmium-exposed workers could be used as biomarkers for suggesting preventive measure.

摘要

目的

本研究旨在探讨镉暴露对从事电镀工作的工人尿 N-乙酰-β-D-氨基葡萄糖苷酶及其同工酶 A 和 B 的影响。

方法

50 名从事电镀镉工作的工人为研究组，选择年龄、性别匹配的在行政部门工作的工人作为对照组。采用无火焰原子吸收分光光度法测定尿镉水平，分光光度法测定尿 N-乙酰-β-D-氨基葡萄糖苷酶及其同工酶 A 和 B。

结果

与对照组相比，研究组尿总 N-乙酰-β-D-氨基葡萄糖苷酶及其同工酶 A 和 B 谱显著增加。尿 N-乙酰-β-D-氨基葡萄糖苷酶及其同工酶 A 和 B 谱水平与尿镉水平呈正相关且显著相关。采用多元回归分析评估尿镉或生活方式混杂因素（年龄、BMI、吸烟和饮酒）对尿 N-乙酰-β-D-氨基葡萄糖苷酶及其同工酶 A 和 B 的影响。分析表明，尿镉水平大于 5μg/g 肌酐、工作年限>15 年、吸烟和体重指数变量的研究对象与尿总 N-乙酰-β-D-氨基葡萄糖苷酶显著相关，但与同工酶 A&B 无关。