Bell Maria C, Schmidt-Grimminger Delf, Patrick Sarah, Ryschon Tim, Linz Laurie, Chauhan Subhash C
Cancer Biology Research Institute, Sanford Research, Sanford School of Medicine, The University of South Dakota, Sioux Falls, SD, USA.
Gynecol Oncol. 2007 Nov;107(2):236-41. doi: 10.1016/j.ygyno.2007.06.007. Epub 2007 Jul 30.
Cervical cancer is the leading gynecological malignancy worldwide, and the incidence of this disease is very high in American Indian women. Infection with the human papillomavirus (HPV) is responsible for more than 95% of cervical squamous carcinomas. Therefore, the main objective of this study was to analyze oncogenic HPV infections in American Indian women residing in the Northern Plains.
Cervical samples were collected from 287 women attending a Northern Plains American Indian reservation outpatient clinic. DNA was extracted from the cervical samples and HPV-specific DNA was amplified by polymerase chain reaction (PCR) using the L1 consensus primer sets. The PCR products were hybridized with the Roche HPV Line Blot assay for HPV genotyping to detect 27 different low- and high-risk HPV genotypes. The Chi-squared test was performed for statistical analysis of the HPV infection and cytology diagnosis data.
Of the total 287 patients, 61 women (21.25%) tested positive for HPV infection. Among all HPV-positive women, 41 (67.2%) were infected with high-risk HPV types. Of the HPV infected women, 41% presented with multiple HPV genotypes. Additionally, of the women infected with oncogenic HPV types, 20 (48.7%) were infected with HPV16 and 18 and the remaining 21 (51.3%) were infected with other oncogenic types (i.e., HPV59, 39, 73). Women infected with oncogenic HPV types had significantly higher (p=0.001) abnormal Papanicolaou smear tests (Pap test) compared to women who were either HPV negative or positive for non-oncogenic HPV types. The incidence of HPV infection was inversely correlated (p<0.05) with the age of the patients, but there was no correlation (p=0.33) with seasonal variation.
In this study, we observed a high prevalence of HPV infection in American Indian women residing on Northern Plains Reservations. In addition, a significant proportion of the oncogenic HPV infections were other than HPV16 and 18.
宫颈癌是全球主要的妇科恶性肿瘤，在美国印第安女性中该疾病的发病率非常高。超过95%的宫颈鳞状细胞癌是由人乳头瘤病毒（HPV）感染引起的。因此，本研究的主要目的是分析居住在大平原北部的美国印第安女性中的致癌性HPV感染情况。
从287名前往大平原北部美国印第安保留地门诊就诊的女性中采集宫颈样本。从宫颈样本中提取DNA，并使用L1共有引物组通过聚合酶链反应（PCR）扩增HPV特异性DNA。PCR产物与罗氏HPV线性印迹检测法杂交进行HPV基因分型，以检测27种不同的低风险和高风险HPV基因型。对HPV感染和细胞学诊断数据进行卡方检验以进行统计分析。
在总共287名患者中，61名女性（21.25%）HPV感染检测呈阳性。在所有HPV阳性女性中，41名（67.2%）感染了高风险HPV类型。在感染HPV的女性中，41%呈现多种HPV基因型。此外，在感染致癌性HPV类型的女性中，20名（48.7%）感染了HPV16和18，其余21名（51.3%）感染了其他致癌类型（即HPV59、39、73）。与HPV阴性或非致癌性HPV类型阳性的女性相比，感染致癌性HPV类型的女性巴氏涂片检查（Pap检查）异常的比例显著更高（p = 0.001）。HPV感染的发生率与患者年龄呈负相关（p < 0.05），但与季节变化无关（p = 0.33）。
在本研究中，我们观察到居住在大平原北部保留地的美国印第安女性中HPV感染的患病率很高。此外，相当一部分致癌性HPV感染并非HPV16和18。
