Størseth Trond R, Kirkvold Ståle, Skjermo Jorunn, Reitan Kjell Inge
Department of Marine Resources Technology, SINTEF Fisheries and Aquaculture, N-7465 Trondheim, Norway.
Carbohydr Res. 2006 Sep 4;341(12):2108-14. doi: 10.1016/j.carres.2006.05.005. Epub 2006 Jun 2.
The chrysolaminaran from the marine diatom Chaetoceros debilis was isolated and characterized by NMR spectroscopy. Cells were harvested in the stationary phase of growth after the medium had been depleted of nitrate when the chrysolaminaran content was expected to be at its highest. The chrysolaminaran was isolated with an yield of 17.5 mg/L, which corresponds to 15.8 pg/cell. 1H NMR indicated that the structure was similar to that of a beta-(1-->3) main chain with beta-(1-->6)-linked side chains. The degree of polymerization was found to be 30, corresponding to a molecular weight of approximately 4900. Thirty-three percent of the residues were found to be beta-(1-->6)-linked branches. The characteristics of the beta-(1-->6) branching were examined by NOESY NMR, which suggested pustulan-like branches, being beta-(1-->6) linked chains connected to the main chain with few branch points. Confirmation of the 1H NMR data was done by 13C-DEPT, TOCSY, COSY and HMQC NMR spectroscopy. The assignment of the resonances of the main beta-(1-->3) and beta-(1-->6) chains is presented. The structure proposed from our analyses is compared against other chrysolaminaran structures.
从海洋硅藻柔弱角毛藻中分离出金藻昆布多糖，并通过核磁共振光谱对其进行了表征。当培养基中的硝酸盐耗尽时，细胞在生长的稳定期收获，此时预计金藻昆布多糖含量最高。金藻昆布多糖的分离产率为17.5 mg/L，相当于每个细胞15.8 pg。1H核磁共振表明，其结构与具有β-(1→6)连接侧链的β-(1→3)主链结构相似。发现聚合度为30，对应分子量约为4900。发现33%的残基为β-(1→6)连接的分支。通过NOESY核磁共振对β-(1→6)分支的特征进行了研究，结果表明其分支类似 pustulan，即β-(1→6)连接的链连接到主链上，分支点较少。通过13C-DEPT、TOCSY、COSY和HMQC核磁共振光谱对1H核磁共振数据进行了确认。给出了主要β-(1→3)和β-(1→6)链的共振归属。将我们分析得出的结构与其他金藻昆布多糖结构进行了比较。
