埃及亚历山大市两家血液透析单位透析液的细菌学监测

Bacteriological monitoring of dialysis fluid in 2 hemodialysis units in Alexandria, Egypt.

作者信息

El-Koraie Ahmed F, Hazzah Walaa A, Abbass Aleya A, El-Shazly Sorya A

机构信息

Department of Internal Medicine and Nephrology, Faculty of Medicine, Alexandria University, 39 Sekina bent, El-Hussein St., Kafr-Abdou, Roshdy, Alexandria, Egypt.

出版信息

Saudi Med J. 2007 Aug;28(8):1234-8.

PMID:17676209

Abstract

OBJECTIVE

To assess the bacteriological quality of dialysis fluid in 2 hemodialysis units in Alexandria, Egypt.

METHODS

A total of 321 samples of hemodialysis fluids, 213 from unit A (a governmental unit), 108 from unit B (a private unit), both under the supervision of ministry of health, were collected from the water treatment system (WTS), treated water, concentrates, and final dialysate from the beginning of March to the end of August 2005. Samples were analyzed for enumeration of the total viable heterotrophic bacteria using the standard pour plate method, and for the determination of the total coliforms (TC) using the presence/absence method. Fifty samples were also examined for endotoxin detection by the Limulus Amoebocyte Lysate assay (LAL), employing the gel clot method.

RESULTS

Percentages of acceptable samples of WTS were 67% from unit A and 66.7% from unit B, while the dialysate samples showed higher acceptability at unit B (86.1%) than unit A (51.7%). Eleven samples were detected as having TC. The LAL assay showed a range of 57-100% of samples exceeded 0.25 EU/ml. Analysis of these results and comparing them to other variables is further discussed.

CONCLUSION

The results demonstrate that hemodialysis centers need monitoring and preventive maintenance in order to ensure renal replacement therapy of good quality.

摘要

目的

评估埃及亚历山大港2个血液透析单位透析液的细菌学质量。

方法

于2005年3月初至8月底，从水处理系统（WTS）、处理后的水、浓缩液和最终透析液中，共采集321份血液透析液样本，其中213份来自A单位（一个政府单位），108份来自B单位（一个私立单位），两个单位均在卫生部的监督下。使用标准倾注平板法分析样本中的总活菌异养菌数量，并使用存在/不存在法测定总大肠菌群（TC）。还采用凝胶凝块法通过鲎试剂法（LAL）对50份样本进行内毒素检测。