Hansen A M, Olsen I L, Holst E, Poulsen O M
Danish National Institute of Occupational Health, Copenhagen.
Ann Occup Hyg. 1991 Dec;35(6):603-11. doi: 10.1093/annhyg/35.6.603.
A high-performance liquid chromatography/fluorescence method using multiple wavelength shift for simultaneous quantification of different PAH compounds was developed. The new method was superior to the methods of DONG and GREENBERG [J. Liquid Chromatogr. 11, 1887-1905 (1988)] and WISE et al. [Polycyclic aromat. Hydrocarb. (in press)] with respect to sensitivity of detection of the majority of 15 PAH compounds, and in particular of naphthalene, acenaphthene, fluorene and benzo(b)fluoranthene. The method of validation analysis employed showed that the new method is in statistical balance meaning that no systematic errors, and only small unsystematic errors, could be demonstrated. Furthermore, the method had a good reproducibility and a high sensitivity.
建立了一种采用多波长切换的高效液相色谱/荧光法同时定量不同多环芳烃化合物的方法。在15种多环芳烃化合物中的大多数，尤其是萘、苊、芴和苯并(b)荧蒽的检测灵敏度方面，该新方法优于董和格林伯格[《液相色谱杂志》11, 1887 - 1905(1988)]以及怀斯等人[《多环芳烃》(即将出版)]的方法。所采用的验证分析方法表明，新方法在统计上是平衡的，这意味着没有系统误差，仅能证明存在较小的非系统误差。此外，该方法具有良好的重现性和高灵敏度。
