Preston D R, Farrah S R
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
Appl Environ Microbiol. 1988 Nov;54(11):2650-4. doi: 10.1128/aem.54.11.2650-2654.1988.
The kinetics of bacteriophage MS2, T2, and f2 adsorption to powdered nitrocellulose and disrupted Seitz S1 filters at pH 7 were determined as a function of temperature. Data from these studies were combined with data produced in a previous study on MS2 adsorption to clay by Stagg et al. (Appl. Environ. Microbiol. 33:385-391, 1977). These workers studied the adsorption of MS2 to bentonite clay as a function of temperature. Data from both this previous study and the current one were used to calculate the thermodynamic parameters of virus adsorption. The results show that adsorption of bacteriophages to the solids tested is a physical process (energy of activation, less than 40 kcal [168 J]/mol) rather than a chemical process (energy of activation, greater than 40 kcal/mol). The free energy of activation showed a high negative correlation (r = -0.904, r2 = 0.817) with the percentage of virus adsorption to the solids tested. The energy of activation was highly negatively correlated with the percentage of virus adsorption to nitrocellulose and clay (r = -0.913, r2 = 0.834) but poorly correlated with the percentage of virus adsorption to disrupted Seitz S1 filters (r = -0.348, r2 = 0.121). In general, under conditions in which the percentage of virus adsorption was low, the energy of activation, the free energy of activation, and the entropy of activation were high. Increasing the percentage of virus adsorbed by changing the adsorbing conditions or changing the adsorbing solid decreased the energy of activation, the free energy of activation, and the entropy of activation.
测定了噬菌体MS2、T2和f2在pH 7时吸附到粉末状硝酸纤维素和破损的赛茨S1滤器上的动力学，并将其作为温度的函数。这些研究的数据与之前Stagg等人关于MS2吸附到黏土上的研究（《应用与环境微生物学》33:385 - 391, 1977年）所产生的数据相结合。这些研究人员研究了MS2吸附到膨润土上的情况，并将其作为温度的函数。将之前这项研究和当前研究的数据用于计算病毒吸附的热力学参数。结果表明，噬菌体吸附到所测试的固体上是一个物理过程（活化能小于40千卡[168焦耳]/摩尔），而非化学过程（活化能大于40千卡/摩尔）。活化自由能与病毒吸附到所测试固体上的百分比呈现出高度负相关（r = -0.904，r² = 0.817）。活化能与病毒吸附到硝酸纤维素和黏土上的百分比高度负相关（r = -0.913，r² = 0.834），但与病毒吸附到破损的赛茨S1滤器上的百分比相关性较差（r = -0.348，r² = 0.121）。一般来说，在病毒吸附百分比低的条件下，活化能、活化自由能和活化熵都很高。通过改变吸附条件或改变吸附固体来增加病毒吸附百分比，会降低活化能、活化自由能和活化熵。
