Kim Myung Kyum, Jung Hee-Young
Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, South Korea.
Int J Syst Evol Microbiol. 2007 Aug;57(Pt 8):1721-1724. doi: 10.1099/ijs.0.64964-0.
A novel strain, designated KP01(T), belonging to the class Sphingobacteria (phylum Bacteroidetes) was isolated from soil in South Korea and was characterized taxonomically using a polyphasic approach. The strain was found to comprise Gram-negative, aerobic, non-motile, non-spore-forming rods. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus Chitinophaga but was clearly separated from established Chitinophaga species. The 16S rRNA gene sequence similarities between KP01(T) and type strains of established Chitinophaga species ranged from 90.3 to 95.7 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C(15 : 0) and C(16 : 1) omega 5c; major hydroxy fatty acid, C(17 : 0) iso 3-OH) supported the affiliation of strain KP01(T) with the genus Chitinophaga. Therefore strain KP01(T) represents a novel species, for which the name Chitinophaga terrae sp. nov. is proposed. The type strain is KP01(T) (=KCTC 12836(T) =LMG 24015(T)).
从韩国土壤中分离出一株新菌株，命名为KP01(T)，属于鞘脂杆菌纲（拟杆菌门），并采用多相分类法对其进行了分类学特征分析。该菌株为革兰氏阴性、需氧、不运动、不产芽孢的杆菌。基于16S rRNA基因序列的系统发育分析表明，该菌株属于噬几丁质菌属，但与已确立的噬几丁质菌种明显不同。KP01(T)与已确立的噬几丁质菌种模式菌株之间的16S rRNA基因序列相似性在90.3%至95.7%之间。表型和化学分类数据（主要甲基萘醌为MK-7；主要脂肪酸为异C(15 : 0)和C(16 : 1) ω5c；主要羟基脂肪酸为C(17 : 0)异3-OH）支持KP01(T)菌株属于噬几丁质菌属。因此，KP01(T)菌株代表一个新物种，提议将其命名为Chitinophaga terrae sp. nov.。模式菌株为KP01(T)（=KCTC 12836(T)=LMG 24015(T)）。
