Lee Dong Wan, Lee Jeong-Eon, Lee Soon Dong
Department of Science Education, Cheju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2830-3. doi: 10.1099/ijs.0.011163-0. Epub 2009 Jul 23.
A Gram-negative, rod-shaped, deep yellow-pigmented bacterium, designated strain CS5-B1(T), was isolated from a soil sample collected from a cliff on Mara Island, south-west of Jeju, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that strain CS5-B1(T) fell within the radiation of the genus Chitinophaga with the closest relative being Chitinophaga japonensis IFO 16041(T) (97.2 % sequence similarity). Levels of 16S rRNA gene sequence similarity between strain CS5-B1(T) and other species of the genus Chitinophaga were 92.9-95.8 %. The dominant menaquinone was MK-7. The polar lipids contained phosphatidylethanolamine, a ninhydrin-positive lipid and an unknown lipid. The major fatty acids were iso-C(15 : 0), C(16 : 1)omega5c, C(16 : 0) and C(18 : 0), with iso-C(15 : 0) 3-OH and iso-C(17 : 0) 3-OH as the dominant hydroxy fatty acids. The G+C content of the DNA was 48.7 mol%. DNA-DNA relatedness between strain CS5-B1(T) and C. japonensis DSM 13484(T) was 5.8-7.9 %. On the basis of the phenotypic and DNA-DNA hybridization data, this bacterium represents a novel species of the genus Chitinophaga, for which the name Chitinophaga rupis sp. nov. is proposed. The type strain is CS5-B1(T) (=KCTC 22170(T)=DSM 21039(T)).
从韩国济州岛西南部马拉岛悬崖采集的土壤样本中分离出一株革兰氏阴性、杆状、深黄色色素细菌，命名为CS5-B1(T)菌株。16S rRNA基因序列比较分析表明，CS5-B1(T)菌株属于噬几丁质菌属辐射范围，与其亲缘关系最近的是日本噬几丁质菌IFO 16041(T)（序列相似性为97.2%）。CS5-B1(T)菌株与噬几丁质菌属其他物种的16S rRNA基因序列相似性水平为92.9 - 95.8%。主要呼吸醌为MK-7。极性脂质含有磷脂酰乙醇胺、一种茚三酮阳性脂质和一种未知脂质。主要脂肪酸为异-C(15 : 0)、C(16 : 1)ω5c、C(16 : 0)和C(18 : 0)，以异-C(15 : 0) 3-OH和异-C(17 : 0) 3-OH为主导羟基脂肪酸。DNA的G+C含量为48.7 mol%。CS5-B1(T)菌株与日本噬几丁质菌DSM 13484(T)的DNA-DNA相关性为5.8 - 7.9%。基于表型和DNA-DNA杂交数据，该细菌代表噬几丁质菌属的一个新物种，为此提出名为岩石噬几丁质菌（Chitinophaga rupis sp. nov.）。模式菌株为CS5-B1(T)（=KCTC 22170(T)=DSM 21039(T)）。
