Vetvicka Vaclav, Volny Tomas, Saraswat-Ohri Sujata, Vashishta Aruna, Vancikova Zuzana, Vetvickova Jana
University of Louisville, Department of Pathology, Louisville, KY 40202, USA.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2007 Jun;151(1):41-6. doi: 10.5507/bp.2007.007.
Recent data showing that glucan elicited defense responses in grapevine and induced protection via induction of resveratrol production led us to evaluate the possible synergetic effects of glucan and resveratrol complex on immune reactions.
We measured phagocytosis using HEMA particles, expression of cell surface markers via fl ow cytometry, expression of cytokines using ELISA, recovery after fluorouracil-induced leucopenia and effects on gene expression via RT-PCR.
Our results showed that both glucan and resveratrol complex stimulated phagocytosis of blood leukocytes, caused increase in surface expression of CD(+) splenocytes and showed higher restoration of spleen recovery after experimentally induced leucopenia. In all these cases, strong synergetic effects were observed. When we measured the effects of these substances on expression level of NF-kappaB2, Cdc42 and Bcl-2 in breast cancer cells, upregulation of Cdc42 expression was evident only using both immunomodulators in combination.
In conclusion, our data suggest significant synergy in stimulation of immune reactions and support further studies of these natural immunomodulators.
最近的数据表明，葡聚糖可引发葡萄中的防御反应，并通过诱导白藜芦醇的产生诱导保护作用，这促使我们评估葡聚糖和白藜芦醇复合物对免疫反应可能产生的协同效应。
我们使用HEMA颗粒测量吞噬作用，通过流式细胞术检测细胞表面标志物的表达，使用酶联免疫吸附测定法检测细胞因子的表达，观察氟尿嘧啶诱导白细胞减少后的恢复情况，并通过逆转录聚合酶链反应检测对基因表达的影响。
我们的结果表明，葡聚糖和白藜芦醇复合物均能刺激血液白细胞的吞噬作用，使CD(+)脾细胞的表面表达增加，并在实验性诱导白细胞减少后显示出更高的脾脏恢复率。在所有这些情况下，均观察到强烈的协同效应。当我们检测这些物质对乳腺癌细胞中NF-κB2、Cdc42和Bcl-2表达水平的影响时，仅在联合使用两种免疫调节剂时，Cdc42表达的上调才明显。
总之，我们的数据表明在刺激免疫反应方面存在显著的协同作用，并支持对这些天然免疫调节剂进行进一步研究。
