Gerecht Sharon, Townsend Seth A, Pressler Heather, Zhu Han, Nijst Christiaan L E, Bruggeman Joost P, Nichol Jason W, Langer Robert
Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Biomaterials. 2007 Nov;28(32):4826-35. doi: 10.1016/j.biomaterials.2007.07.039. Epub 2007 Aug 9.
Encapsulating cells within a polymer matrix creates a three-dimensional (3D) scaffold that may more accurately represent the native microenvironment and cell organization. Here we report a porous scaffold prepared from a photocurable elastomer, poly(glycerolco-sebacate)-acrylate (PGSA). The scaffold porosity, swelling, mass loss, toxicity and mechanical properties, suggest that porous PGSA could be used to support the growth and differentiation of encapsulated cells. Neuroblastoma (NB) and human embryonic stem cells (hESCs) were encapsulated into the matrix and found to adhere to the material and interact with each other within 24h. After 7 days, encapsulated NB cells were found to grow, and form matrix fibrils and tissue. Undifferentiated hESCs proliferated and differentiated in the PGSA scaffold. In vivo experiments showed that both porous scaffolds have similar biocompatibility profiles as non-porous PGSA, but porous PGSA promotes tissue ingrowth, as compared to non-porous PGSA. We therefore propose that porous PGSA scaffolds can provide a logistical template for 3D growth of cells and tissue engineering.
将细胞包裹在聚合物基质中可形成三维（3D）支架，该支架可能更准确地模拟天然微环境和细胞组织。在此，我们报道了一种由光固化弹性体聚（甘油-癸二酸酯）-丙烯酸酯（PGSA）制备的多孔支架。该支架的孔隙率、膨胀率、质量损失、毒性和机械性能表明，多孔PGSA可用于支持被包裹细胞的生长和分化。将神经母细胞瘤（NB）细胞和人类胚胎干细胞（hESCs）包裹在基质中，发现它们在24小时内粘附于材料并相互作用。7天后，发现被包裹的NB细胞生长，并形成基质纤维和组织。未分化的hESCs在PGSA支架中增殖并分化。体内实验表明，与无孔PGSA相比，两种多孔支架具有与无孔PGSA相似的生物相容性，但多孔PGSA促进组织向内生长。因此，我们认为多孔PGSA支架可为细胞的三维生长和组织工程提供一个后勤模板。
