由mrpABCDEFG基因产物形成的复合物，该基因产物构成枯草芽孢杆菌中的一种主要Na⁺/H⁺逆向转运蛋白。
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
作者信息
Kajiyama Yusuke, Otagiri Masato, Sekiguchi Junichi, Kosono Saori, Kudo Toshiaki
机构信息
Environmental Molecular Biology Lab, RIKEN, Wako, Saitama, Japan.
出版信息
J Bacteriol. 2007 Oct;189(20):7511-4. doi: 10.1128/JB.00968-07. Epub 2007 Aug 10.
Abstract
The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na(+)/H(+) antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG gene products by pull-down and blue native polyacrylamide gel electrophoresis analyses. This is the first molecular evidence that the Mrp is a multicomponent antiporter in the cation-proton antiporter 3 family.
摘要
枯草芽孢杆菌Mrp（也称为Sha）是一种由mrpABCDEFG编码的特别不寻常的Na⁺/H⁺逆向转运蛋白。通过对Mrp蛋白进行组氨酸标签标记，我们通过下拉实验和蓝色天然聚丙烯酰胺凝胶电泳分析表明mrpABCDEFG基因产物形成了复合物。这是首个分子证据，证明Mrp是阳离子-质子逆向转运蛋白3家族中的多组分逆向转运蛋白。
相似文献
1
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
J Bacteriol. 2007 Oct;189(20):7511-4. doi: 10.1128/JB.00968-07. Epub 2007 Aug 10.
2
The Na+ transport in gram-positive bacteria defect in the Mrp antiporter complex measured with 23Na nuclear magnetic resonance.
Anal Biochem. 2014 Jan 15;445:80-6. doi: 10.1016/j.ab.2013.10.003. Epub 2013 Oct 15.
3
Catalytic properties of Staphylococcus aureus and Bacillus members of the secondary cation/proton antiporter-3 (Mrp) family are revealed by an optimized assay in an Escherichia coli host.
J Bacteriol. 2007 Apr;189(8):3081-90. doi: 10.1128/JB.00021-07. Epub 2007 Feb 9.
4
The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
Microbiology (Reading). 2009 Jul;155(Pt 7):2137-2147. doi: 10.1099/mic.0.025205-0. Epub 2009 Apr 23.
5
Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans.
J Bacteriol. 2016 Dec 28;199(2). doi: 10.1128/JB.00662-16. Print 2017 Jan 15.
6
Functional involvement of membrane-embedded and conserved acidic residues in the ShaA subunit of the multigene-encoded Na+/H+ antiporter in Bacillus subtilis.
Biochim Biophys Acta. 2006 May;1758(5):627-35. doi: 10.1016/j.bbamem.2006.04.012. Epub 2006 Apr 26.
7
Functional role of the MrpA- and MrpD-homologous protein subunits in enzyme complexes evolutionary related to respiratory chain complex I.
Biochim Biophys Acta. 2014 Jan;1837(1):178-85. doi: 10.1016/j.bbabio.2013.09.012. Epub 2013 Oct 1.
8
NhaK, a novel monovalent cation/H+ antiporter of Bacillus subtilis.
Arch Microbiol. 2005 Sep;183(6):411-20. doi: 10.1007/s00203-005-0011-6. Epub 2005 Oct 13.
9
mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis.
J Bacteriol. 1999 Apr;181(8):2394-402. doi: 10.1128/JB.181.8.2394-2402.1999.
10
Putative paired small multidrug resistance family proteins PsmrAB, the homolog of YvdSR, actually function as a novel two-component Na(+)/H(+) antiporter.
FEMS Microbiol Lett. 2013 Jan;338(1):31-8. doi: 10.1111/1574-6968.12008. Epub 2012 Oct 8.
引用本文的文献
1
Genomic Evidence for the Recycling of Complex Organic Carbon by Novel Clades in Deep-Sea Sediments.
mSystems. 2022 Jun 28;7(3):e0007722. doi: 10.1128/msystems.00077-22. Epub 2022 Apr 18.
2
Transcriptome analysis provides new insights into the tolerance and reduction of Lysinibacillus fusiformis 15-4 to hexavalent chromium.
Appl Microbiol Biotechnol. 2021 Oct;105(20):7841-7855. doi: 10.1007/s00253-021-11586-3. Epub 2021 Sep 21.
3
Comparative Genomics of Reveals What Makes a Cosmopolitan Bacterium.
mSystems. 2021 Aug 31;6(4):e0038321. doi: 10.1128/mSystems.00383-21. Epub 2021 Jul 20.
4
Structure of the Mrp complex reveals molecular mechanism of this giant bacterial sodium proton pump.
Proc Natl Acad Sci U S A. 2020 Dec 8;117(49):31166-31176. doi: 10.1073/pnas.2006276117. Epub 2020 Nov 23.
5
Mechanism of TonB-dependent transport system in Halomonas alkalicola CICC 11012s in response to alkaline stress.
Extremophiles. 2021 Jan;25(1):39-49. doi: 10.1007/s00792-020-01209-6. Epub 2020 Oct 30.
6
The complete genome sequence of Rhodobaca barguzinensis alga05 (DSM 19920) documents its adaptation for life in soda lakes.
Extremophiles. 2018 Nov;22(6):839-849. doi: 10.1007/s00792-018-1041-8. Epub 2018 Jul 18.
7
The Lysine 299 Residue Endows the Multisubunit Mrp1 Antiporter with Dominant Roles in Na Resistance and pH Homeostasis in Corynebacterium glutamicum.
Appl Environ Microbiol. 2018 May 1;84(10). doi: 10.1128/AEM.00110-18. Print 2018 May 15.
8
Mrp Antiporters Have Important Roles in Diverse Bacteria and Archaea.
Front Microbiol. 2017 Nov 23;8:2325. doi: 10.3389/fmicb.2017.02325. eCollection 2017.
9
Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans.
J Bacteriol. 2016 Dec 28;199(2). doi: 10.1128/JB.00662-16. Print 2017 Jan 15.
10
The Blueprint of a Minimal Cell: MiniBacillus.
Microbiol Mol Biol Rev. 2016 Sep 28;80(4):955-987. doi: 10.1128/MMBR.00029-16. Print 2016 Dec.
本文引用的文献
1
Tricine-SDS-PAGE.
Nat Protoc. 2006;1(1):16-22. doi: 10.1038/nprot.2006.4.
2
Functional involvement of membrane-embedded and conserved acidic residues in the ShaA subunit of the multigene-encoded Na+/H+ antiporter in Bacillus subtilis.
Biochim Biophys Acta. 2006 May;1758(5):627-35. doi: 10.1016/j.bbamem.2006.04.012. Epub 2006 Apr 26.
3
Transposon disruption of the complex I NADH oxidoreductase gene (snoD) in Staphylococcus aureus is associated with reduced susceptibility to the microbicidal activity of thrombin-induced platelet microbicidal protein 1.
J Bacteriol. 2006 Jan;188(1):211-22. doi: 10.1128/JB.188.1.211-222.2006.
4
A shaE deletion mutant showed lower Na+ sensitivity compound to other deletion mutants in the Bacillus subtilis sodium/hydrogen antiporter (Sha) system.
J Biosci Bioeng. 2003;95(3):306-9. doi: 10.1016/s1389-1723(03)80035-x.
5
The Mrp system: a giant among monovalent cation/proton antiporters?
Extremophiles. 2005 Oct;9(5):345-54. doi: 10.1007/s00792-005-0451-6. Epub 2005 Jun 25.
6
Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis.
FEMS Microbiol Lett. 2004 Mar 12;232(1):93-9. doi: 10.1016/S0378-1097(04)00037-0.
7
Mfold web server for nucleic acid folding and hybridization prediction.
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15. doi: 10.1093/nar/gkg595.
8
Essential Bacillus subtilis genes.
Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4678-83. doi: 10.1073/pnas.0730515100. Epub 2003 Apr 7.
9
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading). 2002 Nov;148(Pt 11):3539-3552. doi: 10.1099/00221287-148-11-3539.
10
Oligomeric state of membrane transport proteins analyzed with blue native electrophoresis and analytical ultracentrifugation.
J Mol Biol. 2002 Apr 5;317(4):591-600. doi: 10.1006/jmbi.2002.5416.
Zotero 插件