由mrpABCDEFG基因产物形成的复合物，该基因产物构成枯草芽孢杆菌中的一种主要Na⁺/H⁺逆向转运蛋白。
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
作者信息
Kajiyama Yusuke, Otagiri Masato, Sekiguchi Junichi, Kosono Saori, Kudo Toshiaki
机构信息
Environmental Molecular Biology Lab, RIKEN, Wako, Saitama, Japan.
出版信息
J Bacteriol. 2007 Oct;189(20):7511-4. doi: 10.1128/JB.00968-07. Epub 2007 Aug 10.
Abstract
The Bacillus subtilis Mrp (also referred to as Sha) is a particularly unusual Na(+)/H(+) antiporter encoded by mrpABCDEFG. Using His tagging of Mrp proteins, we showed complex formation by the mrpABCDEFG gene products by pull-down and blue native polyacrylamide gel electrophoresis analyses. This is the first molecular evidence that the Mrp is a multicomponent antiporter in the cation-proton antiporter 3 family.
摘要
枯草芽孢杆菌Mrp（也称为Sha）是一种由mrpABCDEFG编码的特别不寻常的Na⁺/H⁺逆向转运蛋白。通过对Mrp蛋白进行组氨酸标签标记，我们通过下拉实验和蓝色天然聚丙烯酰胺凝胶电泳分析表明mrpABCDEFG基因产物形成了复合物。这是首个分子证据，证明Mrp是阳离子-质子逆向转运蛋白3家族中的多组分逆向转运蛋白。
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2
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3
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5
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10
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