Diamanti-Kandarakis Evanthia, Piperi Christina, Korkolopoulou Penelope, Kandaraki Eleni, Levidou Georgia, Papalois Apostolos, Patsouris Efstratios, Papavassiliou Athanasios G
First Department of Internal Medicine, Endocrine section, Medical School, University of Athens, Athens, Greece.
J Mol Med (Berl). 2007 Dec;85(12):1413-20. doi: 10.1007/s00109-007-0246-6. Epub 2007 Aug 11.
The aim of the present study was to investigate whether dietary advanced glycation end-products (AGEs) can be detected in the ovarian tissue of normal female rats and whether they can affect their metabolic or hormonal profile. Sixty normal rats (20 animals in each group) were randomly assigned to regular diet, either high (H-AGE) or low (L-AGE) in AGE content for 6 months. H-AGE rats demonstrated higher levels of fasting glucose (P < 0.001), insulin (P < 0.069), and serum AGEs (P < 0.001) than control and L-AGE rats. Additionally, the H-AGE group showed increased AGE localization in the theca interna cells of the ovarian tissue compared to control/L-AGE rats (P = 0.003). Furthermore, increased receptor for AGE (RAGE) staining was also observed in granulosa cells compared to control/L-AGE samples (P = 0.038). In the H-AGE group, plasma testosterone was higher than in control rats (P < 0.001) and in the L-AGE group (P < 0.001). However, H-AGE rats did not exhibit higher body weight compared with normal (P = 0.118) and L-AGE-fed rats (P = 0.35). These results demonstrate for the first time that administration of high AGE diet in female rats for a prolonged period is associated with increased deposition of AGEs in the theca cells and of RAGE in the granulosa and theca interna cells of the ovarian tissue compared with the corresponding ovarian compartments of the control and L-AGE-fed animals. The metabolic alterations in conjuction with the increased deposition in ovarian tissues of dietary glycotoxins and elevated levels of testosterone in H-AGE-fed animals compared to the controls suggest an impact of environmental factors on ovarian tissue and these findings need further exploration.
本研究的目的是调查正常雌性大鼠的卵巢组织中是否能检测到膳食晚期糖基化终产物（AGEs），以及它们是否会影响其代谢或激素水平。60只正常大鼠（每组20只）被随机分为常规饮食组，其AGE含量分别为高（H-AGE）或低（L-AGE），持续6个月。与对照组和L-AGE组大鼠相比，H-AGE组大鼠的空腹血糖（P < 0.001）、胰岛素（P < 0.069）和血清AGEs（P < 0.001）水平更高。此外，与对照组/L-AGE组大鼠相比，H-AGE组卵巢组织的内膜细胞中AGE定位增加（P = 0.003）。此外，与对照组/L-AGE组样本相比，颗粒细胞中AGE受体（RAGE）染色也增加（P = 0.038）。在H-AGE组中，血浆睾酮高于对照组大鼠（P < 0.001）和L-AGE组（P < 0.001）。然而，H-AGE组大鼠与正常饮食组（P = 0.118）和L-AGE组喂养的大鼠相比，体重并未增加（P = 0.35）。这些结果首次表明，与对照组和L-AGE组喂养动物的相应卵巢区域相比，长期给雌性大鼠喂食高AGE饮食会导致AGEs在卵巢组织的内膜细胞中沉积增加，以及RAGE在颗粒细胞和内膜细胞中沉积增加。与对照组相比，H-AGE组喂养动物的代谢改变以及膳食糖毒素在卵巢组织中的沉积增加和睾酮水平升高表明环境因素对卵巢组织有影响，这些发现需要进一步探索。
