Stepanović Srdjan, Vuković Dragana, Hola Veronika, Di Bonaventura Giovanni, Djukić Slobodanka, Cirković Ivana, Ruzicka Filip
Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, Belgrade, Serbia.
APMIS. 2007 Aug;115(8):891-9. doi: 10.1111/j.1600-0463.2007.apm_630.x.
The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.
本文描述了微量滴定板中生物膜形成定量分析所涉及的所有步骤的详细信息。所提出的方案纳入了通过直接经验以及对生物膜产生检测方法的分析而获得的关于葡萄球菌生物膜产生评估的信息。所获得的结果应能简化微量滴定板中生物膜形成的定量分析，并使其在不同实验室之间更可靠且具有可比性。
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