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微量滴定板中生物膜的定量:葡萄球菌生物膜产生评估的测试条件概述及实用建议

Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci.

作者信息

Stepanović Srdjan, Vuković Dragana, Hola Veronika, Di Bonaventura Giovanni, Djukić Slobodanka, Cirković Ivana, Ruzicka Filip

机构信息

Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, Belgrade, Serbia.

出版信息

APMIS. 2007 Aug;115(8):891-9. doi: 10.1111/j.1600-0463.2007.apm_630.x.

DOI:10.1111/j.1600-0463.2007.apm_630.x
PMID:17696944
Abstract

The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.

摘要

本文描述了微量滴定板中生物膜形成定量分析所涉及的所有步骤的详细信息。所提出的方案纳入了通过直接经验以及对生物膜产生检测方法的分析而获得的关于葡萄球菌生物膜产生评估的信息。所获得的结果应能简化微量滴定板中生物膜形成的定量分析,并使其在不同实验室之间更可靠且具有可比性。

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