Ma Chao, Xiao Sheng-yuan, Li Zhen-guo, Wang Wei, Du Li-jun
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
J Chromatogr A. 2007 Sep 21;1165(1-2):39-44. doi: 10.1016/j.chroma.2007.07.060. Epub 2007 Aug 1.
HPLC-DAD-MS was utilized to investigate the phytochemical constituents in ethanolic extract of Ananas comosus L. leaves (EEACL) responsible for antidiabetic, antihyperlipidemic and antioxidative effects. Eight phenylpropane diglycerides, together with two hydroxycinnamic acids, three hydroxycinnamoyl quinic acids, four phenylpropane monoglycerides, three flavones and six phenylpropanoid glycosides were detected, and their proposed structures were elucidated based on HPLC retention time, UV and MS profiles. Meanwhile, a new HPLC-DAD-MS method was established for the identification and characterization of phenylpropane diglycerides in natural plants.
采用高效液相色谱-二极管阵列检测器-质谱联用（HPLC-DAD-MS）技术，对凤梨叶乙醇提取物（EEACL）中具有抗糖尿病、抗高血脂和抗氧化作用的植物化学成分进行了研究。检测到8种苯丙烷二甘油酯，以及2种羟基肉桂酸、3种羟基肉桂酰奎宁酸、4种苯丙烷单甘油酯、3种黄酮和6种苯丙烷糖苷，并根据HPLC保留时间、紫外光谱和质谱图对其推测结构进行了阐明。同时，建立了一种新的HPLC-DAD-MS方法，用于天然植物中苯丙烷二甘油酯的鉴定和表征。
