Helyes Z, Pozsgai G, Börzsei R, Németh J, Bagoly T, Márk L, Pintér E, Tóth G, Elekes K, Szolcsányi J, Reglodi D
Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Pécs, Hungary.
Peptides. 2007 Sep;28(9):1847-55. doi: 10.1016/j.peptides.2007.07.001. Epub 2007 Jul 7.
Inhibitory actions of pituitary adenylate cyclase activating polypeptide (PACAP) have been described on cellular/vascular inflammatory components, but there are few data concerning its role in neurogenic inflammation. In this study we measured PACAP-like immunoreactivity with radioimmunoassay in the rat plasma and showed a two-fold elevation in response to systemic stimulation of capsaicin-sensitive sensory nerves by resiniferatoxin, but not after local excitation of cutaneous afferents. Neurogenic plasma extravasation in the plantar skin induced by intraplantar capsaicin or resiniferatoxin, as well as carrageenan-induced paw edema were significantly diminished by intraperitoneal PACAP-38. In summary, these results demonstrate that PACAP is released from activated capsaicin-sensitive afferents into the systemic circulation. It diminishes acute pure neurogenic and mixed-type inflammatory reactions via inhibiting pro-inflammatory mediator release and/or by acting at post-junctional targets on the vascular endothelium.
垂体腺苷酸环化酶激活多肽（PACAP）对细胞/血管炎症成分具有抑制作用，但其在神经源性炎症中的作用数据较少。在本研究中，我们用放射免疫分析法测定了大鼠血浆中PACAP样免疫反应性，结果显示，在用树脂毒素全身性刺激辣椒素敏感感觉神经后，其水平升高了两倍，但在局部刺激皮肤传入神经后则没有升高。腹腔注射PACAP-38可显著减轻足底注射辣椒素或树脂毒素诱导的足底皮肤神经源性血浆外渗，以及角叉菜胶诱导的爪肿胀。总之，这些结果表明，PACAP从激活的辣椒素敏感传入神经释放到体循环中。它通过抑制促炎介质释放和/或作用于血管内皮的后接头靶点，减轻急性单纯神经源性和混合型炎症反应。
