Malmström Johan, Lee Hookeun, Aebersold Ruedi
Institute for Molecular Systems Biology, ETH Zürich, Switzerland.
Curr Opin Biotechnol. 2007 Aug;18(4):378-84. doi: 10.1016/j.copbio.2007.07.005. Epub 2007 Aug 14.
Mass spectrometry, specifically the analysis of complex peptide mixtures by liquid chromatography and tandem mass spectrometry (shotgun proteomics) has been at the centre of proteomics research for the past decade. To overcome some of the fundamental limitations of the approach, including its limited sensitivity and high degree of redundancy, new proteomic workflows are being developed. Among these, targeting methods in which specific peptides are selectively isolated, identified and quantified are particularly promising. Here we summarize recent incremental advances in shotgun proteomic methods and outline emerging targeted workflows. The development of the target-driven approaches with their ability to detect and quantify identical, non-redundant sets of proteins in multiple repeat analyses will be crucially important for the application of proteomics to biomarker discovery and validation, and to systems biology research.
在过去十年中，质谱分析，特别是通过液相色谱和串联质谱对复杂肽混合物进行分析（鸟枪法蛋白质组学）一直是蛋白质组学研究的核心。为了克服该方法的一些基本局限性，包括其有限的灵敏度和高度冗余性，新的蛋白质组学工作流程正在不断开发。其中，能够选择性分离、鉴定和定量特定肽段的靶向方法尤其具有前景。在此，我们总结了鸟枪法蛋白质组学方法的最新进展，并概述了新兴的靶向工作流程。目标驱动方法能够在多次重复分析中检测和定量相同的、无冗余的蛋白质组，其发展对于蛋白质组学在生物标志物发现与验证以及系统生物学研究中的应用至关重要。
