Farca A M, Nebbia P, Re G
Department of Animal Pathology, University of Torino, Italy.
Vet Res Commun. 1994;18(1):1-6. doi: 10.1007/BF01839255.
The in vitro synergistic effects of combinations of EDTA-tromethamine and five antimicrobial agents (ampicillin, cephalexin, oxytetracycline, streptomycin and sulphadimethoxine) on three clinically isolated Gram-positive bacteria (Staphylococcus aureus, Staphylococcus hominis and Streptococcus faecium) were investigated. The bacteria had been isolated from three cases of canine otitis resistant to beta-lactam antibiotic therapy. The antimicrobial activity was evaluated by measuring the minimal inhibitory concentration for the antibiotics alone or in combination with EDTA-tromethamine. EDTA-tromethamine potentiated the activity of cefalexin against S. aureus and S. hominis, of oxytetracycline against S. aureus and S. faecium and of streptomycin against S. faecium. No significant effects were noted on the activity of oxytetracycline against S. hominis. The remaining combinations gave a slight synergistic effect. As previously shown for Gram-negative resistant bacteria, these data suggest that the association of EDTA-tromethamine and appropriate antibiotic therapy may be useful to overcome persistent infections of soft tissues in domestic animals.
研究了乙二胺四乙酸-氨丁三醇与五种抗菌剂（氨苄青霉素、头孢氨苄、土霉素、链霉素和磺胺二甲氧嘧啶）联合使用对三种临床分离的革兰氏阳性菌（金黄色葡萄球菌、人葡萄球菌和粪肠球菌）的体外协同作用。这些细菌是从三例对β-内酰胺类抗生素治疗耐药的犬中耳炎病例中分离出来的。通过测量单独使用抗生素或与乙二胺四乙酸-氨丁三醇联合使用时的最低抑菌浓度来评估抗菌活性。乙二胺四乙酸-氨丁三醇增强了头孢氨苄对金黄色葡萄球菌和人葡萄球菌的活性、土霉素对金黄色葡萄球菌和粪肠球菌的活性以及链霉素对粪肠球菌的活性。未观察到土霉素对人葡萄球菌活性有显著影响。其余组合产生轻微的协同作用。如先前对革兰氏阴性耐药菌的研究所示，这些数据表明乙二胺四乙酸-氨丁三醇与适当的抗生素治疗联合使用可能有助于克服家畜软组织的持续性感染。
