Effect of Xiaobailing decoction on melanocytes in vitro.

OBJECTIVE

To observe the effect of xiaobailing decoction ([Chinese characters: see text) on murine melanocytes in vitro and to explore the mechanism of xiaobailing Decoction in the treatment of vitiligo.

METHODS

B-16F10 murine melanoma cells were cultured in 1640 medium and treated respectively with different concentrations (1 mg/ml, 2 mg/ml, 3 mg/ml) of the Chinese drug xiaobailing Decoction and its main components, the drugs for replenishing the kidney-yang, and the drugs for nourishing blood and activating blood circulation, etc. for 7 days. MMT assay was used to determine the proliferation of B-16F10 murine melanoma cells. NaOH cleavage assay was adopted to measure the melanogenesis of melanocytes.

RESULTS

xiaobailing Decoction, the drugs for replenishing the kidney-yang and the drugs for nourishing blood and activating blood circulation at different concentrations significantly improved the proliferation of B-16F10 murine melanoma cells from the 3rd day to the 5th day (P < 0.05), with xiaobailing Decoction at the concentrations of 1 mg/ml having the most distinct action on promoting the proliferation of melanocytes on the 3rd day (P < 0.001); And the drugs for replenishing the kidney-yang at the concentrations of 2 mg/ml and 3 mg/ml and the drugs for nourishing blood and activating blood circulation at 3 mg/ml significantly increased melanogenesis of melanocytes (P < 0.05).

CONCLUSION

Xiaobailing Decoction can promote melanocytic proliferation and melanogenesis in vitro, and it is indicated that the drugs for replenishing the kidney-yang and the drugs for nourishing blood and activating blood circulation play an important role in treating vitiligo.