Lotterer E, Ramaker J, Lüdtke F E, Tegeler R, Geletneky J V, Bauer F E
Department of Clinical Pharmacology, University of Goettingen, Bundesrepublik Deutschland.
Z Gastroenterol. 1991 Nov;29(11):590-4.
A 13C-urea breath test for detection of Helicobacter pylori infection was validated in 306 patients. Breath samples (four, two or one) were taken at various time points within 30 minutes after a motility inhibiting liquid test meal with citric acid followed by 75 mg of 13C-urea. The 13CO2/12 CO2-ratio (delta-value) was measured using isotope ratio mass spectrometry and the recovery of tracer in the exhaled breath was calculated (UBT-value). In 172 patients a comparison of established reference methods (culture, CLO test, and Fuchsin staining) with the 4 point breath analysis for detection of Helicobacter pylori showed high values of quality parameters (greater than 80% for sensitivity, specificity, positive and negative predictive value), independent of expression of the results as delta-value or as UBT-value. In 134 patients a reduction of samples to one single breath sample taken 30 minutes after ingestion of the tracer showed no significant differences in the quality parameters when compared with the standard 4 point breath analysis. This indicates that the analysis of a single breath sample is suitable for detection of Helicobacter pylori status in man.
一项用于检测幽门螺杆菌感染的13C尿素呼气试验在306例患者中得到验证。在食用含柠檬酸的动力抑制液体试验餐并服用75毫克13C尿素后30分钟内的不同时间点采集呼气样本（4份、2份或1份）。使用同位素比率质谱法测量13CO2/12CO2比率（δ值），并计算呼出气体中示踪剂的回收率（UBT值）。在172例患者中，将既定的参考方法（培养、CLO试验和品红染色）与用于检测幽门螺杆菌的4点呼气分析进行比较，结果显示质量参数值较高（灵敏度、特异性、阳性和阴性预测值均大于80%），且结果以δ值或UBT值表示时均不受影响。在134例患者中，与标准的4点呼气分析相比，将样本减少为摄入示踪剂后30分钟采集的单一呼气样本，质量参数无显著差异。这表明分析单一呼气样本适用于检测人体中的幽门螺杆菌状态。
