Mizuno Noriyoshi, Shiba Hideki, Xu Wan-peng, Inui Takafumi, Fujita Tsuyoshi, Kajiya Mikihito, Takeda Katsuhiro, Hasegawa Naohiko, Kawaguchi Hiroyuki, Kurihara Hidemi
Department of Periodontal Medicine, Division of Frontier Medical Science, Programs for Biomedical Research, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3, Kasumi, Hiroshima 734-8553, Japan.
Cell Biol Int. 2007 Dec;31(12):1462-9. doi: 10.1016/j.cellbi.2007.06.012. Epub 2007 Jul 17.
Neurotrophins (NTs) are expressed during tooth development. However, little is known about a role of NTs in differentiation of pulp cells into mineralizing cells. In this study, mRNA expressions of hard tissue-related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)-3 and NT-4/5 increased the mRNA levels of dentin sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone morphogenetic protein-2 and mineral deposition in cultures of HP cells. The increased levels and manners varied, depending on the concentrations of NTs and hard-tissue related protein tested. On the other hand, only NGF significantly stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs characteristically regulate hard-tissue related protein expression, calcification and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.
神经营养因子（NTs）在牙齿发育过程中表达。然而，关于NTs在牙髓细胞分化为矿化细胞中的作用知之甚少。在本研究中，检测了人牙髓（HP）细胞培养物中硬组织相关蛋白的mRNA表达、钙化和增殖情况。神经生长因子（NGF）、脑源性神经营养因子（BDNF）、神经营养因子（NT）-3和NT-4/5可提高HP细胞培养物中牙本质涎磷蛋白、碱性磷酸酶、骨桥蛋白、I型胶原和骨形态发生蛋白-2的mRNA水平以及矿物质沉积。增加的水平和方式因NTs浓度和所检测的硬组织相关蛋白而异。另一方面，只有NGF能显著刺激HP细胞培养物中的DNA合成。这些发现表明，NTs可特异性调节牙髓细胞中硬组织相关蛋白的表达、钙化和增殖。NTs可能加速牙髓细胞分化。
