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1
Plasmid-determined alcohol dehydrogenase activity in alkane-utilizing strains of Pseudomonas putida.恶臭假单胞菌利用烷烃菌株中质粒决定的乙醇脱氢酶活性
J Bacteriol. 1976 May;126(2):794-8. doi: 10.1128/jb.126.2.794-798.1976.
2
Regulation of alkane oxidation in Pseudomonas putida.恶臭假单胞菌中烷烃氧化的调控
J Bacteriol. 1975 Aug;123(2):546-56. doi: 10.1128/jb.123.2.546-556.1975.
3
Fractionation of inducible alkane hydroxylase activity in Pseudomonas putida and characterization of hydroxylase-negative plasmid mutations.恶臭假单胞菌中诱导型烷烃羟化酶活性的分级分离及羟化酶阴性质粒突变的表征
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4
N-Alkane oxidation enzymes of a pseudomonad.一种假单胞菌的正构烷烃氧化酶。
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5
Genetic regulation of octane dissimilation plasmid in Pseudomonas.假单胞菌中辛烷异化质粒的遗传调控
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8
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Physiological function of the Pseudomonas putida PpG6 (Pseudomonas oleovorans) alkane hydroxylase: monoterminal oxidation of alkanes and fatty acids.恶臭假单胞菌PpG6（食油假单胞菌）烷烃羟化酶的生理功能：烷烃和脂肪酸的单末端氧化
J Bacteriol. 1975 Apr;122(1):93-8. doi: 10.1128/jb.122.1.93-98.1975.

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1
Biochemical analysis of recombinant AlkJ from Pseudomonas putida reveals a membrane-associated, flavin adenine dinucleotide-dependent dehydrogenase suitable for the biosynthetic production of aliphatic aldehydes.恶臭假单胞菌重组AlkJ的生化分析表明，它是一种与膜相关的、黄素腺嘌呤二核苷酸依赖性脱氢酶，适用于脂肪醛的生物合成生产。
Appl Environ Microbiol. 2014 Apr;80(8):2468-77. doi: 10.1128/AEM.04297-13. Epub 2014 Feb 7.
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Plasmid Involvement in Acyclic Isoprenoid Metabolism by Pseudomonas putida.质粒参与假单胞菌的非环异戊二烯代谢。
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3
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4
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5
Bacterial formation and metabolism of 6-hydroxyhexanoate: evidence of a potential role for omega-oxidation.6-羟基己酸的细菌形成与代谢：ω-氧化潜在作用的证据
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6
Reversal by DNA amplifications of an unusual mutation blocking alkane and alcohol utilization in Pseudomonas putida.恶臭假单胞菌中阻断烷烃和醇类利用的异常突变通过DNA扩增实现逆转。
Mol Gen Genet. 1984;197(3):384-91. doi: 10.1007/BF00329933.
7
Molecular cloning and characterization of sequences from the regulatory cluster of the Pseudomonas plasmid alk system.假单胞菌质粒alk系统调控簇序列的分子克隆与特性分析
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8
Alcohol dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecane and hexadecanol metabolism.不动杆菌属菌株HO1-N中的乙醇脱氢酶：在十六烷和十六醇代谢中的作用
J Bacteriol. 1985 Dec;164(3):1017-24. doi: 10.1128/jb.164.3.1017-1024.1985.
9
Fertility factors in Pseudomonas putida: selection and properties of high-frequency transfer and chromosome donors.恶臭假单胞菌中的育性因子：高频转移和染色体供体的筛选及特性
J Bacteriol. 1979 Jan;137(1):28-34. doi: 10.1128/jb.137.1.28-34.1979.
10
Transposition of a beta-lactamase locus from RP1 into Pseudomonas putida degradative plasmids.β-内酰胺酶基因座从RP1转位至恶臭假单胞菌降解性质粒中。
J Bacteriol. 1977 Feb;129(2):809-14. doi: 10.1128/jb.129.2.809-814.1977.

本文引用的文献

1
Paraffin Oxidation in Pseudomonas aeruginosa II. Gross Fractionation of the Enzyme System into Soluble and Particulate Components.铜绿假单胞菌中的石蜡氧化 II. 酶系统粗分为可溶性和颗粒性组分
J Bacteriol. 1970 Dec;104(3):1065-73. doi: 10.1128/jb.104.3.1065-1073.1970.
2
HYDROCARBON OXIDATION BY A BACTERIAL ENZYME SYSTEM. II. COFACTOR REQUIREMENTS FOR OCTANOL FORMATION FROM OCTANE.细菌酶系统催化的烃类氧化。II. 由辛烷生成辛醇的辅因子需求
Biochemistry. 1963 Sep-Oct;2:1155-9. doi: 10.1021/bi00905a043.
3
Extraction and properties of alcohol dehydrogenase from Pseudomonas aeruginosa.铜绿假单胞菌中乙醇脱氢酶的提取及性质
Biochim Biophys Acta. 1963 May 7;73:1-6. doi: 10.1016/0006-3002(63)90353-6.
4
Genetic fusion of incompatible plasmids in Pseudomonas.假单胞菌中不兼容质粒的基因融合
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1641-4. doi: 10.1073/pnas.70.6.1641.
5
Genetic regulation of octane dissimilation plasmid in Pseudomonas.假单胞菌中辛烷异化质粒的遗传调控
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1137-40. doi: 10.1073/pnas.70.4.1137.
6
Separation and characterization of long-chain alcohol dehydrogenase isoenzymes from Pseudomonas aeruginosa.铜绿假单胞菌长链醇脱氢酶同工酶的分离与鉴定
Biochim Biophys Acta. 1972 Jul 13;276(1):31-42. doi: 10.1016/0005-2744(72)90005-8.
7
Occurrence of inducible and NAD(P)-independent primary alcohol dehydrogenases in an alkane-oxidizing Pseudomonas.一株烷烃氧化假单胞菌中可诱导的和不依赖NAD(P)的初级醇脱氢酶的出现。
Antonie Van Leeuwenhoek. 1969;35(3):344-60. doi: 10.1007/BF02219154.
8
Induction of alkane hydroxylase proteins by unoxidized alkane in Pseudomonas putida.恶臭假单胞菌中未氧化烷烃对烷烃羟化酶蛋白的诱导作用。
J Bacteriol. 1975 Aug;123(2):759-60. doi: 10.1128/jb.123.2.759-760.1975.
9
Regulation of alkane oxidation in Pseudomonas putida.恶臭假单胞菌中烷烃氧化的调控
J Bacteriol. 1975 Aug;123(2):546-56. doi: 10.1128/jb.123.2.546-556.1975.
10
Physiological function of the Pseudomonas putida PpG6 (Pseudomonas oleovorans) alkane hydroxylase: monoterminal oxidation of alkanes and fatty acids.恶臭假单胞菌PpG6（食油假单胞菌）烷烃羟化酶的生理功能：烷烃和脂肪酸的单末端氧化
J Bacteriol. 1975 Apr;122(1):93-8. doi: 10.1128/jb.122.1.93-98.1975.

恶臭假单胞菌利用烷烃菌株中质粒决定的乙醇脱氢酶活性

Plasmid-determined alcohol dehydrogenase activity in alkane-utilizing strains of Pseudomonas putida.

作者信息

Benson S, Shapiro J

出版信息

J Bacteriol. 1976 May;126(2):794-8. doi: 10.1128/jb.126.2.794-798.1976.

DOI:10.1128/jb.126.2.794-798.1976
PMID:177405
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC233215/
Abstract

We have identified an alcohol dehydrogenase activity in Pseudomonas putida strains carrying the CAM-OCT degradative plasmid that were grown on octane. The activity is nicotinamide adenine dinucleotide independent, sediments at 48,000 x g, and shows 20-fold greater activity with octanol rather than butanol as substrate. The enzyme is inducible by unoxidized alkane and is present only in strains that have the OCT plasmid genes for alkane degradation with a wild-type alcO locus. No analogous chromosomal dehydrogenase could be detected. Wild-type and actanol-negative mutants (alcA-) without plasmids both contain a constitutive nicotinamide adenine dinucleotide-linked soluble alcohol dehydrogenase activity. This means that alcA- mutants are cryptic for octanol oxidation and suggests that the particulate plasmid-coded alcohol dehydrogenase activity is active on surface- or membrane-bound substrate.

摘要

我们在携带CAM - OCT降解质粒且以辛烷为生长底物的恶臭假单胞菌菌株中鉴定出了一种乙醇脱氢酶活性。该活性不依赖烟酰胺腺嘌呤二核苷酸，在48,000 x g下可沉淀，以辛醇而非丁醇作为底物时活性高20倍。该酶可被未氧化的烷烃诱导，且仅存在于具有用于烷烃降解的OCT质粒基因及野生型alcO位点的菌株中。未检测到类似的染色体脱氢酶。无质粒的野生型和辛醇阴性突变体（alcA-）均含有组成型烟酰胺腺嘌呤二核苷酸连接的可溶性乙醇脱氢酶活性。这意味着alcA-突变体对辛醇氧化是隐性的，表明颗粒状质粒编码的乙醇脱氢酶活性作用于表面或膜结合的底物。