β-内酰胺酶基因座从RP1转位至恶臭假单胞菌降解性质粒中。
Transposition of a beta-lactamase locus from RP1 into Pseudomonas putida degradative plasmids.
作者信息
Benedik M, Fennewald M, Shapiro J
出版信息
J Bacteriol. 1977 Feb;129(2):809-14. doi: 10.1128/jb.129.2.809-814.1977.
The beta-lactamase gene from the RP1 plasmid transposes into at least two Pseudomonas putida degradative plasmids. Donor strains that carry RP1 (bla+ tet+ aphA+) and a degradative plasmid yield transconjugants that have only the bla+ marker of RP1. This occurs in up to 80% of all bla+ transconjugants. Segregation of the bla+ marker requires the presence of a degradative plasmid in the donor and is only observed in transconjugants that have received degradative markers. The bla+ tet aphA transconjugants show 100% linkage of bla+ to degradative markers in conjugation,transduction, and transformation crosses. A transduction cross of an (RP1), (SAL) donor shows that 8% of all SAL plasmids also carry the transposed bla+ marker. Tn401 is the name we assign to the bla+ transposon from RP1 observed in Pseudomonas. Its identity with the RP1 bla+ transposon observed in Escherichia coli is not known. In four cases, Tn401 has inserted into the camphor genes of the CAM-OCT plasmid.
来自RP1质粒的β-内酰胺酶基因可转座至至少两种恶臭假单胞菌的降解性质粒中。携带RP1（bla⁺ tet⁺ aphA⁺）和一个降解性质粒的供体菌株产生的转接合子仅具有RP1的bla⁺标记。这在所有bla⁺转接合子中发生率高达80%。bla⁺标记的分离需要供体中存在降解性质粒，并且仅在获得降解标记的转接合子中观察到。bla⁺ tet aphA转接合子在接合、转导和转化杂交中显示bla⁺与降解标记100%连锁。一个（RP1）、（SAL）供体的转导杂交显示，所有SAL质粒中有8%也携带转座的bla⁺标记。Tn401是我们赋予在假单胞菌中观察到的来自RP1的bla⁺转座子的名称。它与在大肠杆菌中观察到的RP1 bla⁺转座子的同一性尚不清楚。在四个案例中，Tn401已插入到CAM - OCT质粒的樟脑基因中。
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