Hermann M, Garg G K, Gunsalus I C
J Bacteriol. 1979 Jan;137(1):28-34. doi: 10.1128/jb.137.1.28-34.1979.
The octane plasmid (OCT) in Pseudomonas putida strains has been shown to be transferred at low frequency. However, bacteria which had newly received this plasmid showed a transient increase in donor ability. Using Octane+ P. putida as the donor, the transfer of most chromosomal markers was shown to be independent of OCT transfer, whereas the mobilization of the octanoate catabolism genes (octanoic and acetate) was dependent on OCT plasmid transfer. The presence of a fertility factor termed FPo has been postulated to explain these results. Strains carrying only this fertility factor have been obtained from strains carrying both OCT and FPo plasmids. Strains in which the OCT plasmid was transferred at high frequencies have also been isolated, and chromosome mobilization by OCT and FPo has been compared. A different gradient of transmission by OCT and FPo has been observed. It has also been shown that chromosome transfer by OCT was dependent on the bacterial recombination system, whereas the chromosome transfer by FPo was unaffected by the presence of a rec mutation in the donor strain.
恶臭假单胞菌菌株中的辛烷质粒（OCT）已被证明能以低频率转移。然而，新获得该质粒的细菌显示出供体能力的短暂增加。以辛烷+恶臭假单胞菌作为供体，大多数染色体标记的转移被证明与OCT转移无关，而辛酸分解代谢基因（辛酸和乙酸）的移动则依赖于OCT质粒转移。据推测，存在一种名为FPo的致育因子来解释这些结果。仅携带这种致育因子的菌株已从同时携带OCT和FPo质粒的菌株中获得。也分离出了OCT质粒高频转移的菌株，并比较了OCT和FPo对染色体的移动情况。观察到OCT和FPo有不同的传递梯度。还表明，OCT介导的染色体转移依赖于细菌重组系统，而FPo介导的染色体转移不受供体菌株中rec突变的影响。
