Chakrabarty A M
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1641-4. doi: 10.1073/pnas.70.6.1641.
The genes specifying enzymes responsible for the degradation of camphor and octane occur on transmissible plasmids in Pseudomonas putida strain PpG1 and P. oleovorans. Since the presence of the plasmids is vital for the oxidative metabolism of camphor or octane (by the cells) in the absence of other carbon sources, such naturally occurring, energy-generating plasmids have been designated as degradative plasmids. The two degradative plasmids, CAM and OCT, are incompatible with each other and cannot coexist in the same cell. By the use of UV irradiation and suitable selection techniques, it has been possible to fuse these two plasmids so they become part of the same replicon and coexist. Such a technique might be useful in introducing several degradative pathways in the same cell.
在恶臭假单胞菌菌株PpG1和食油假单胞菌中，负责樟脑和辛烷降解的酶的编码基因存在于可传递质粒上。由于在没有其他碳源的情况下，质粒的存在对于（细胞）对樟脑或辛烷的氧化代谢至关重要，这种天然存在的、产生能量的质粒被称为降解质粒。两种降解质粒，CAM和OCT，彼此不相容，不能共存于同一细胞中。通过紫外线照射和合适的筛选技术，已能够将这两种质粒融合，使其成为同一复制子的一部分并共存。这种技术可能有助于在同一细胞中引入几种降解途径。
