采用高效薄层色谱-二苯基苦味酰基自由基（HPTLC-DPPH）法对姜黄主要姜黄素类化合物进行分离及自由基清除活性研究。

Separation and free radical-scavenging activity of major curcuminoids of Curcuma longa using HPTLC-DPPH method.

作者信息

Pozharitskaya Olga N, Ivanova Svetlana A, Shikov Alexander N, Makarov Valery G

机构信息

Interregional Center Adaptogen, 47/5, Piskarevsky pr., 195067, St-Petersburg, Russia.

出版信息

Phytochem Anal. 2008 May-Jun;19(3):236-43. doi: 10.1002/pca.1035.

DOI:10.1002/pca.1035

PMID:17929240

Abstract

A direct HPTLC assay was developed for the determination of total curcuminoids and three individual curcuminoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin. In addition, a new procedure was developed to separate and quantitative the free radical-scavenging activity of individual compounds from the rhizome of Curcuma longa L. (Zingiberaceae) based on the combination of HPTLC with a diode array detector (DAD) and post chromatographic DPPH() radical derivatisation. It was established that both individual curcuminoids and the extract of C. longa were capable of scavenging DPPH() radicals. From the estimated ID(50) values, it can be seen that the order of activity was curcumin > demethoxycurcumin > bisdemethoxycurcumin >> ascorbic acid. However, the ID(50) values of curcuminoids were not significantly different. The data indicates the presence of a synergistic mechanism of antiradical activity of curcuminoids.

摘要

建立了一种直接高效薄层色谱（HPTLC）分析法，用于测定总姜黄素类化合物以及三种单一姜黄素类化合物，即姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素。此外，还开发了一种新方法，基于高效薄层色谱与二极管阵列检测器（DAD）联用以及色谱后二苯代苦味酰基自由基（DPPH()）衍生化，来分离和定量来自姜黄（姜科）根茎中单一化合物的自由基清除活性。结果表明，单一姜黄素类化合物和姜黄提取物均具有清除DPPH()自由基的能力。从估算的半数抑制浓度（ID(50)）值可以看出，活性顺序为姜黄素>去甲氧基姜黄素>双去甲氧基姜黄素>>抗坏血酸。然而，姜黄素类化合物的ID(50)值并无显著差异。数据表明姜黄素类化合物存在抗自由基活性的协同机制。