Younes R N, Vydelingum N A, Derooij P, Scognamiglio F, Andrade L, Posner M C, Brennan M F
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
HPB Surg. 1991;5(1):35-48. doi: 10.1155/1991/27457.
We examined the effect of prolonged bile duct obstruction, and subsequent biliary decompression, on biochemical and metabolic parameters, using a reversible jaundice model in male Fischer 344 rats. The animals were studied after biliary obstruction for varying periods (4 days, one week, and two weeks) and following decompression. They were sacrificed one or two weeks following decompression. All the rats were compared to sham operated, pair-fed, controls. Obstructive jaundice rapidly increased bilirubin, liver enzymes, serum free fatty acid, and triglyceride levels. Glucose levels were significantly decreased in the jaundice rats compared to their pair-fed controls. Only after two weeks of jaundice was significant hypoalbuminemia observed. Following decompression, all biochemical and metabolic values gradually returned to normal levels, except for albumin. Hypoalbuminemia was not reversed within the two-week post-decompression period. The rats jaundiced for two weeks had significantly higher mortality, compared to the other groups. We conclude that prolonged jaundice adversely affects the metabolic capacity of the rats, with albumin concentration being markedly decreased, and that biliary decompression could not reverse completely all the alterations seen with cholestasis, especially following two weeks of bile duct obstruction.
我们使用雄性Fischer 344大鼠的可逆性黄疸模型，研究了长期胆管梗阻及随后的胆道减压对生化和代谢参数的影响。在胆管梗阻不同时间段（4天、1周和2周）及减压后对动物进行研究。在减压后1周或2周处死动物。将所有大鼠与假手术、配对饲养的对照组进行比较。梗阻性黄疸迅速升高胆红素、肝酶、血清游离脂肪酸和甘油三酯水平。与配对饲养的对照组相比，黄疸大鼠的血糖水平显著降低。仅在黄疸2周后观察到明显的低白蛋白血症。减压后，除白蛋白外，所有生化和代谢值逐渐恢复到正常水平。低白蛋白血症在减压后两周内未得到逆转。与其他组相比，黄疸2周的大鼠死亡率显著更高。我们得出结论，长期黄疸对大鼠的代谢能力有不利影响，白蛋白浓度明显降低，并且胆道减压不能完全逆转胆汁淤积所见的所有改变，尤其是在胆管梗阻2周后。
