Doi K, Kojima A, Akiyama Y, Kato S
Natl Inst Anim Health Q (Tokyo). 1976 Spring;16(1):16-24.
Pathogenicity for chicks of the MSB-1 line, a cell line derived from the tumorous tissue of a chick with Marek's disease (MD) and established by Akiyama & Kato, was studied. Five groups, including a control one, of 20 chicks each were inoculated with 1 X 10(3), 1 X 10(4), 1 X 10(5), 1 X 10(6) and no cells of a 180-day culture of the cell line at one day of age. They were housed all together in an isolation unit. An attempt was first made successfully to isolate MD virus (MDV) directly in culture of kidney cells 3 weeks after inoculation. Horizontal infection was first detected 4 weeks after inoculation. From 3 weeks after inoculation on, the disease with almost the same clinical and pathological pictures as the infection with a virulent strain of MDV showed a high incidence. Morbidity was closely related to the number of MSB-1 line cells inoculated. Parenchymal destruction was conspicuous in the central lymphoid organs of four chicks given the largest number of MSB-1 line cells and sacrificed in extremis about 4 weeks after inoculation. Establishment of MD in chicks inoculated with MSB-1 line cells carrying MDV genome seemed to be initiated under the circumstances where the line cells which had come into contact with susceptible cells in the peritoneal cavity released virulent MDV per se. Then host chicks might be infected with MDV and suffer from MD at a high rate. There was no great difference in oncogenic potential between MSB-1 line cells cultivated in vitro for 180 days and virulent MDV serially passaged through one-day-old chicks.
对MSB - 1细胞系（一种从患有马立克氏病（MD）的雏鸡肿瘤组织中分离并由秋山和加藤建立的细胞系）对雏鸡的致病性进行了研究。包括一个对照组在内的五组雏鸡，每组20只，在一日龄时分别接种1×10³、1×10⁴、1×10⁵、1×10⁶个该细胞系180天培养物的细胞以及不接种细胞。它们一起饲养在一个隔离单元中。接种3周后首次成功尝试在肾细胞培养物中直接分离马立克氏病病毒（MDV）。接种4周后首次检测到水平感染。从接种3周后开始，出现了与感染MDV强毒株几乎相同临床和病理表现的疾病，发病率很高。发病率与接种的MSB - 1细胞系细胞数量密切相关。接种了最多数量MSB - 1细胞系细胞并在接种后约4周濒死处死的4只雏鸡的中央淋巴器官实质破坏明显。接种携带MDV基因组的MSB - 1细胞系细胞的雏鸡发生MD，似乎是在腹腔中与易感细胞接触的细胞系细胞释放出有毒力的MDV本身的情况下开始的。然后宿主雏鸡可能被MDV感染并以高发病率患MD。在体外培养180天的MSB - 1细胞系细胞与通过一日龄雏鸡连续传代的强毒MDV之间，致癌潜力没有很大差异。
