非组蛋白的去磷酸化会特异性地改变重构染色质中的基因转录模式。
Dephosphorylation of nonhistone proteins specifically alters the pattern of gene transcription in reconstituted chromatin.
作者信息
Kleinsmith L J, Stein J, Stein G
出版信息
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1174-8. doi: 10.1073/pnas.73.4.1174.
Abstract
A nonhistone phosphatase devoid of detectable protease activity has been purified from nuclear sap by chromatography on DEAE-Sephadex. After linkage to an insoluble agarose matrix, this enzyme was used to dephosphorylate nonhistone proteins obtained from S-phase HeLa cells. Chromatin reconstituted with these dephosphorylated proteins exhibited roughly a 50% reduction in the overall number of initiation sites available for transcription when compared to controls. Specific measurements of transcription of histone genes with use of a complementary DNA probe showed that genes coding for histones are preferentially inhibited after nonhistone dephosphorylation. These results provide the first direct support for the theory that phosphorylation of nonhistone proteins is involved in regulating the availability of individual genes for transcription.
摘要
一种没有可检测到的蛋白酶活性的非组蛋白磷酸酶已通过在DEAE-葡聚糖凝胶上的色谱法从核液中纯化出来。与不溶性琼脂糖基质连接后，这种酶被用于使从S期海拉细胞获得的非组蛋白去磷酸化。与对照相比，用这些去磷酸化蛋白质重构的染色质在可用于转录的起始位点总数上大致减少了50%。使用互补DNA探针进行的组蛋白基因转录的特异性测量表明，在非组蛋白去磷酸化后，编码组蛋白的基因受到优先抑制。这些结果为非组蛋白蛋白质的磷酸化参与调节单个基因转录可用性的理论提供了首个直接支持。
相似文献
1
Dephosphorylation of nonhistone proteins specifically alters the pattern of gene transcription in reconstituted chromatin.非组蛋白的去磷酸化会特异性地改变重构染色质中的基因转录模式。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1174-8. doi: 10.1073/pnas.73.4.1174.
2
Activation of in vitro histone gene transcription from Hela S3 chromatin by S-phase nonhistone chromosomal proteins.S期非组蛋白染色体蛋白对Hela S3染色质体外组蛋白基因转录的激活作用。
Biochemistry. 1976 Jul 27;15(15):3296-30. doi: 10.1021/bi00660a020.
3
Effect of histones and nonhistone chromosomal proteins on the transcription of histone genes from HeLaS3 cell DNA.组蛋白和非组蛋白染色体蛋白对HeLaS3细胞DNA中组蛋白基因转录的影响。
Biochemistry. 1976 Jul 27;15(15):3291-5. doi: 10.1021/bi00660a019.
4
Activation of histone gene transcription by nonhistone chromosomal phosphoproteins.非组蛋白染色体磷蛋白对组蛋白基因转录的激活作用。
Science. 1976 Oct 22;194(4263):428-31. doi: 10.1126/science.982025.
5
Role of nonhistone chromosomal proteins in the restriction of mitotic chromatin template activity.非组蛋白染色体蛋白在有丝分裂染色质模板活性限制中的作用。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2918-21. doi: 10.1073/pnas.69.10.2918.
6
Nonhistone proteins control gene expression in reconstituted chromatin.非组蛋白在重构染色质中控制基因表达。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):5057-61. doi: 10.1073/pnas.71.12.5057.
7
Role of nonhistone chromosomal proteins in the regulation of histone gene expression.非组蛋白染色体蛋白在组蛋白基因表达调控中的作用。
Cancer Res. 1976 Nov;36(11 Pt. 2):4307-18.
8
Activation of histone gene transcription by nonhistone chromosomal proteins in WI-38 human diploid fibroblasts.非组蛋白染色体蛋白对WI-38人二倍体成纤维细胞中组蛋白基因转录的激活作用。
Proc Natl Acad Sci U S A. 1977 Jan;74(1):173-7. doi: 10.1073/pnas.74.1.173.
9
Involvement of Nonhistone Chromosomal Proteins in Transcriptional Activity of Chromatin during Wheat Germination.非组蛋白染色体蛋白在小麦萌发过程中对染色质转录活性的影响
Plant Physiol. 1979 Jun;63(6):1016-21. doi: 10.1104/pp.63.6.1016.
10
Regulation of cell cycle stage-specific transcription of histone genes from chromatin by non-histone chromosomal proteins.非组蛋白染色体蛋白对染色质中组蛋白基因细胞周期阶段特异性转录的调控。
Nature. 1975 Oct 30;257(5529):764-7. doi: 10.1038/257764a0.
引用本文的文献
1
Auxin-induced changes in chromosomal protein phosphorylation in wounded potato tuber parenchyma.伤诱导的马铃薯块茎薄壁组织染色体蛋白磷酸化的变化。
Plant Mol Biol. 1981 Mar;1(1):5-17. doi: 10.1007/BF00023010.
2
3
Chicken liver non-histone protein fraction repressing DNA transcription in vitro.鸡肝非组蛋白组分在体外抑制DNA转录
Mol Biol Rep. 1984 Jul;10(1):13-8. doi: 10.1007/BF00775148.
4
Phosphorylation-dephosphorylation of nuclear proteins during infection (Q fever).感染（Q热）期间核蛋白的磷酸化-去磷酸化
Infect Immun. 1984 Jan;43(1):14-20. doi: 10.1128/iai.43.1.14-20.1984.
5
Phosphorylation of nuclear and DNA-binding proteins in proliferating and quiescent mammalian cells.增殖和静止的哺乳动物细胞中核蛋白及DNA结合蛋白的磷酸化作用
Biochem J. 1981 Jan 15;194(1):193-207. doi: 10.1042/bj1940193.
6
Cyclic nucleotide-independent protein kinases bound to cytoplasmic and nuclear polyribosomes in non-infected and adenovirus-infected HeLa cells.在未感染和腺病毒感染的HeLa细胞中，与细胞质和核多核糖体结合的不依赖环核苷酸的蛋白激酶。
Biochem J. 1980 Jul 1;189(1):143-52. doi: 10.1042/bj1890143.
7
Incorporation of various amino acids into non-histone chromatin protein fractions of spleen cells of mice immunized with IgG.用免疫球蛋白G免疫的小鼠脾细胞非组蛋白染色质蛋白组分中各种氨基酸的掺入情况。
Mol Cell Biochem. 1981 Jun 9;37(1):13-9. doi: 10.1007/BF02355883.
8
Regulation of specific genes during the cell cycle. Utilization of homologous cDNAs and cloned sequences for studying histone gene expression in human cells.细胞周期中特定基因的调控。利用同源cDNA和克隆序列研究人类细胞中的组蛋白基因表达。
Cell Biophys. 1980 Dec;2(4):291-314. doi: 10.1007/BF02785095.
9
Non-histone chromatin proteins in beef thyroid: distinct phosphorylation patterns of several protein kinases.牛肉甲状腺中的非组蛋白染色质蛋白：几种蛋白激酶的不同磷酸化模式
Mol Cell Biochem. 1990 Jun 25;95(2):139-46. doi: 10.1007/BF00219972.
10
Adenovirus type 2 coded single-stranded DNA binding protein: in vivo phosphorylation and modification.2型腺病毒编码的单链DNA结合蛋白：体内磷酸化与修饰
J Virol. 1977 May;22(2):402-11. doi: 10.1128/JVI.22.2.402-411.1977.
本文引用的文献
1
Role of non-histone components in determining organ specificity of rabbit chromatins.非组蛋白成分在决定兔染色质器官特异性中的作用。
FEBS Lett. 1970 Aug 17;9(4):242-244. doi: 10.1016/0014-5793(70)80366-0.
2
Nuclear phosphoproteins. I. Isolation and characterization of a phosphoprotein fraction from calf thymus nuclei.核磷蛋白。I. 从小牛胸腺核中分离和鉴定一种磷蛋白组分。
Biochim Biophys Acta. 1969 Feb 4;175(1):123-35.
3
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
4
Sequence-specific interaction of DNA and chromosomal protein.DNA与染色体蛋白的序列特异性相互作用。
J Mol Biol. 1969 Jan;39(2):351-64. doi: 10.1016/0022-2836(69)90322-2.
5
Phosphoprotein metabolism in isolated lymphocyte nuclei.分离的淋巴细胞核中的磷蛋白代谢
Proc Natl Acad Sci U S A. 1966 May;55(5):1182-9. doi: 10.1073/pnas.55.5.1182.
6
The synthesis of acidic chromosomal proteins during the cell cycle of HeLa S-3 cells. I. The accelerated accumulation of acidic residual nuclear protein before the initiation of DNA replication.海拉S-3细胞周期中酸性染色体蛋白的合成。I. DNA复制起始前酸性残留核蛋白的加速积累。
J Cell Biol. 1972 Feb;52(2):292-307. doi: 10.1083/jcb.52.2.292.
7
Degradation of chromosomal proteins during dissociation and reconstitution of chromatin.染色质解离和重组过程中染色体蛋白的降解
Biochem Biophys Res Commun. 1974 Apr 8;57(3):740-6. doi: 10.1016/0006-291x(74)90608-1.
8
Transcription of chromatin in vitro.体外染色质转录
J Mol Biol. 1973 Jun 25;77(2):237-54. doi: 10.1016/0022-2836(73)90334-3.
9
Role of nonhistone chromosomal proteins in the restriction of mitotic chromatin template activity.非组蛋白染色体蛋白在有丝分裂染色质模板活性限制中的作用。
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2918-21. doi: 10.1073/pnas.69.10.2918.
10
Nonhistone chromosomal proteins and gene regulation.非组蛋白染色体蛋白与基因调控。
Science. 1974 Mar 1;183(4127):817-24. doi: 10.1126/science.183.4127.817.
Zotero 插件