Leong A S, Duncis C G
Pathology. 1986 Apr;18(2):222-5. doi: 10.3109/00313028609059463.
This report describes the use of microwave irradiation for the rapid fixation of large biopsy specimens. Two hundred and twelve fresh large specimens subjected to non-ionizing irradiation in a domestic microwave oven (600 watt output, 2450 MHz) for 4-9 min were rendered sufficiently firm to be dissected and sampled immediately without the need for any formalin fixation. The tissues hardened in this manner remained pliable, retained much of their natural colour and were free of noxious fumes. Lymph nodes became opaque and firm and their pink-tan colour contrasted with the bright yellow of adipose tissue, making them easily dissectable. This procedure, combined with a further 4-7 min exposure to microwaves of the trimmed tissue blocks (in batches of 40), completed fixation and allowed the specimens to be processed on a 3 h 15 min cycle in an autoprocessor which did not contain formalin.
本报告描述了利用微波辐射对大型活检标本进行快速固定的方法。212个新鲜的大型标本在一台家用微波炉（输出功率600瓦，频率2450兆赫）中接受非电离辐射4至9分钟后，变得足够坚硬，可立即进行解剖和取样，无需任何福尔马林固定。以这种方式硬化的组织仍保持柔韧，保留了大部分天然颜色，且无有害烟雾。淋巴结变得不透明且坚硬，其粉褐色与脂肪组织的亮黄色形成对比，使其易于解剖。此程序，再加上将修整后的组织块（每批40个）进一步暴露于微波中4至7分钟，完成固定，并使标本能够在不含福尔马林的自动组织处理仪中以3小时15分钟的周期进行处理。
