Staebell M, Soll D R
J Gen Microbiol. 1985 Jun;131(6):1467-80. doi: 10.1099/00221287-131-6-1467.
The infectious yeast Candida albicans is capable of growing in either a budding or mycelium form, depending upon the pH of the supporting medium. By monitoring the position of polylysine-coated beads firmly attached to the wall of growing cells, the zones of expansion for the surface of the cell wall have been mapped for the alternative growth forms. Both spatial and temporal differences are demonstrated to exist. During roughly the first two-thirds of bud growth, a very small, highly active apical zone accounts for roughly 70% of surface expansion. The remaining 30% is due to general expansion. When a bud reaches approximately two-thirds of its final surface area, the apical zone shuts down, and subsequent expansion is completed by the general mechanism. During mycelial growth, at least 90% of expansion is due to a small, highly active apical growth zone, and less than 10% is due to the general mechanism. In contrast to budding cells, the apical zone of the growing mycelium never shuts down as long as growth continues in the mycelial form. These distinct temporal and spatial differences in expansion are considered in terms of the regulation of alternative phenotypes in Candida.
感染性酵母白色念珠菌能够根据支持培养基的pH值以出芽或菌丝体形式生长。通过监测牢固附着在生长细胞壁上的聚赖氨酸包被珠的位置，已绘制出细胞壁表面在两种不同生长形式下的扩展区域。结果表明，在空间和时间上都存在差异。在芽生长的大约前三分之二时间里，一个非常小但高度活跃的顶端区域约占表面扩展的70%。其余30%则是由于整体扩展。当一个芽达到其最终表面积的大约三分之二时，顶端区域停止活动，随后的扩展由整体机制完成。在菌丝体生长过程中，至少90%的扩展是由于一个小的、高度活跃的顶端生长区域，不到10%是由于整体机制。与出芽细胞不同，只要菌丝体形式的生长持续，生长中的菌丝体的顶端区域就不会停止活动。这些在扩展方面明显的时间和空间差异，从白色念珠菌替代表型的调控角度进行了探讨。
