Walker Christopher M, Ko Gwangpyo
University of Texas Health Science Center at Houston, Houston, TX, USA.
Environ Sci Technol. 2007 Aug 1;41(15):5460-5. doi: 10.1021/es070056u.
Ultraviolet (UV) germicidal air disinfection is an engineering method used to control the airborne transmission of pathogenic microorganisms in high-risk settings. Despite the recent emergence of respiratory viral pathogens such as SARS and avian influenza viruses, UV disinfection of pathogenic viral aerosols has not been examined. Hence, we characterized the UV disinfection of viral aerosols using the bacteriophage MS2, adenovirus, and coronavirus. Our objectives were to characterize the effect of nebulization and air sampling on the survival of important viral pathogens, quantitatively characterize and estimate the UV susceptibility of pathogenic viral aerosols, and evaluate the effect of relative humidity (RH) on the susceptibility of viral aerosols, to 254 nm UV-C. The viruses were aerosolized into an experimental chamber using a six-jet Collison nebulizer, exposed to 254 nm UV, and sampled using an AGI-30 liquid impinger. Both the MS2 and adenovirus aerosols were very resistant to UV air disinfection, with a reduction of less than 1 logarithm in viable viral aerosols at a UV dose of 2608 microW s/cm2. The susceptibility of coronavirus aerosols was 7-10 times that of the MS2 and adenovirus aerosols. Unlike bacterial aerosols, there was no significant protective effect of high RH on UV susceptibility of the tested viral aerosols. We confirmed that the UV disinfection rate differs greatly between viral aerosols and viruses suspended in liquid.
紫外线（UV）空气杀菌消毒是一种用于控制高风险环境中病原微生物空气传播的工程方法。尽管近期出现了如SARS和禽流感病毒等呼吸道病毒病原体，但尚未对病原性病毒气溶胶的紫外线消毒进行研究。因此，我们使用噬菌体MS2、腺病毒和冠状病毒对病毒气溶胶的紫外线消毒进行了表征。我们的目标是表征雾化和空气采样对重要病毒病原体存活的影响，定量表征和估算病原性病毒气溶胶的紫外线敏感性，并评估相对湿度（RH）对病毒气溶胶对254 nm紫外线C敏感性的影响。使用六喷口碰撞雾化器将病毒雾化到实验室内，使其暴露于254 nm紫外线，并使用AGI - 30液体冲击器进行采样。MS2和腺病毒气溶胶对紫外线空气消毒都具有很强的抗性，在紫外线剂量为2608微瓦·秒/平方厘米时，活病毒气溶胶的减少量小于1个对数级。冠状病毒气溶胶的敏感性是MS2和腺病毒气溶胶的7 - 10倍。与细菌气溶胶不同，高相对湿度对所测试病毒气溶胶的紫外线敏感性没有显著的保护作用。我们证实，病毒气溶胶和悬浮在液体中的病毒之间的紫外线消毒率差异很大。
