Menini Teresita, Heck Caleb, Schulze John, de Mejia Elvira, Gugliucci Alejandro
Glycation, Oxidation and Disease Laboratory, Division of Basic Medical Sciences, Touro University-California, Mare Island, Vallejo, CA 94592, USA.
Planta Med. 2007 Sep;73(11):1141-7. doi: 10.1055/s-2007-981585. Epub 2007 Sep 7.
Paraoxonase 1 (PON-1), an antioxidant enzyme carried mainly by HDL, has been shown to display cardioprotective effects. In this study, we investigated whether the polyphenol-rich beverage mate, obtained from extract of Ilex paraguariensis (IP), prevented the loss of PON-1 activity from HDL during oxidant stress. The peroxide radical generator 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) induced time- and dose-dependent oxidation of HDL, as measured by lipoperoxide content, which is accompanied by a parallel decrease in the activity of PON-1 (p<0.001). IP extract (2-20 microL/mL) afforded time- and concentration-dependent inhibition of the oxidation, with preservation of apoA-I structure and PON-1 activity. Healthy volunteers drank either 0.5 L of IP extract, 0.5 L of coffee and milk or nothing. PON-1 activity increased an average of 10% above the changes seen when the intake was coffee and milk (p<0.05). In conclusion, we demonstrate that IP extract may, to some extent, prevent the loss of the antiatherogenic function of HDL afforded by PON-1 when the particle is under oxidant stress.
对氧磷酶1（PON-1）是一种主要由高密度脂蛋白（HDL）携带的抗氧化酶，已被证明具有心脏保护作用。在本研究中，我们调查了从巴拉圭冬青（IP）提取物中获得的富含多酚的饮品马黛茶是否能在氧化应激期间防止HDL中PON-1活性的丧失。过氧化物自由基发生器2,2-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）诱导HDL发生时间和剂量依赖性氧化，通过脂质过氧化物含量测定，同时PON-1活性平行下降（p<0.001）。IP提取物（2-20微升/毫升）对氧化具有时间和浓度依赖性抑制作用，同时保留载脂蛋白A-I结构和PON-1活性。健康志愿者分别饮用0.5升IP提取物、0.5升咖啡和牛奶或不饮用任何饮品。与饮用咖啡和牛奶时相比，PON-1活性平均增加了10%（p<0.05）。总之，我们证明当HDL颗粒处于氧化应激状态时，IP提取物在一定程度上可能防止PON-1所赋予的HDL抗动脉粥样硬化功能的丧失。
