拉雷亚树：对巨噬细胞和低密度脂蛋白的抗炎和抗氧化作用。

Larrea divaricata: anti-inflammatory and antioxidant effects of on macrophages and low density lipoproteins.

机构信息

Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Buenos Aires, Argentina.

Cátedra de Farmacognosia, Facultad de farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

出版信息

BMC Complement Med Ther. 2022 Mar 23;22(1):84. doi: 10.1186/s12906-022-03547-8.

BACKGROUND

The oxidized low density lipoprotein (ox-LDL) contributes to inflammation and oxidative stress through the activation of macrophages under hyperglycemia contributing to the development of diabetes mellitus and to atherosclerosis. Plants are a source of effective and innocuous antioxidants. Larrea divaricata Cav. (Zygophyllaceae) is used in Argentina folk medicine for its anti-inflammatory properties.

METHODS

The aim of this work was to study the antioxidant and anti-inflammatory effects of the aqueous extract (AE) of L. divaricata on macrophages under glucose stimulation and on human LDL and HDL particles under free radical generators.

RESULTS

AE reduced the lipid peroxidation (17%), nitric oxide (NO) (47-50%), tumor necrosis factor-α (TNF-α) (32%) and free radicals (50%) induced by glucose on macrophages. Also prevented HDL nitration (28%), thus preserving its function and structure and inhibited LDL oxidation. The effect on the nitrosative stress was mainly driven by nordihydroguaiaretic acid (NDGA).

CONCLUSIONS

These results suggest a potential usefulness of AE as an adjuvant phytotherapy in patients with diabetes mellitus and atherosclerosis.

背景

氧化型低密度脂蛋白（ox-LDL）通过在高血糖下激活巨噬细胞引发炎症和氧化应激，从而促进糖尿病和动脉粥样硬化的发展。植物是有效且无毒的抗氧化剂的来源。阿根廷民间医学用 Larrea divaricata Cav.（蒺藜科）治疗炎症。

方法

本研究的目的是研究 L. divaricata 的水提物（AE）在葡萄糖刺激下对巨噬细胞的抗氧化和抗炎作用，以及在自由基生成剂下对人 LDL 和 HDL 颗粒的作用。

结果

AE 降低了由葡萄糖诱导的巨噬细胞中的脂质过氧化（17%）、一氧化氮（NO）（47-50%）、肿瘤坏死因子-α（TNF-α）（32%）和自由基（50%）。AE 还可防止 HDL 硝化（28%），从而保持其功能和结构，并抑制 LDL 氧化。对硝化应激的影响主要归因于 nordihydroguaiaretic acid（NDGA）。