Kim Chul Young, Lee Hee Ju, Lee Mi Kyeong, Ahn Mi-Jeong, Kim Jinwoong
Natural Product Research Center, KIST Gangneung Institute, Daejeon-dong, Gangneung, Gangwon-do, Korea.
J Sep Sci. 2007 Nov;30(16):2693-7. doi: 10.1002/jssc.200700168.
Flavanone glycosides were successfully separated from the crude extract of Poncirus trifoliata by preparative centrifugal partition chromatography with a two-phase solvent system composed of ethyl acetate-acetonitrile-water (3:2:5, v/v/v). Naringin (50.0 mg), neoponcirin (16.8 mg), and poncirin (71.9 mg) were purified from the 524 mg crude extract in only one step. The purities of the isolated compounds were determined to be over 90% by HPLC analysis and their structures were elucidated by (1)H NMR, (13)C NMR, and ESI-MS.
采用由乙酸乙酯 - 乙腈 - 水（3:2:5，v/v/v）组成的两相溶剂系统，通过制备型离心分配色谱法成功地从枳壳粗提取物中分离出黄酮苷。仅一步就从524毫克粗提取物中纯化出柚皮苷（50.0毫克）、新橙皮苷（16.8毫克）和枳橙苷（71.9毫克）。通过高效液相色谱分析测定分离出的化合物纯度超过90%，并通过¹H NMR、¹³C NMR和电喷雾电离质谱对其结构进行了阐明。
