Tastekin Nurettin, Aydogdu Nurettin, Dokmeci Dikmen, Usta Ufuk, Birtane Murat, Erbas Hakan, Ture Mevlut
Department of Physical Medicine and Rehabilitation, Faculty of Medicine, University of Trakya, Edirne, Turkey.
Pharmacol Res. 2007 Oct;56(4):303-10. doi: 10.1016/j.phrs.2007.07.008. Epub 2007 Aug 3.
Free radicals play an important role in the pathophysiology of adjuvant arthritis. The purpose of this study was to assess the efficacy of L-carnitine (LC) and alpha-lipoic acid (alpha-LA) which are known to have antioxidant effects, in the treatment of adjuvant arthritis. Arthritis model was created by the administration of complete Freund's adjuvant (CFA) in 32 of 40 male Sprague-Dawley rats. The rats were divided into five groups. Rats in Group I served as controls and received 0.1 ml kg(-1) saline. Group II received only 0.1 ml of CFA and served as the CFA-control for the other groups. Groups III-V, after being injected with CFA, were treated with LC, alpha-LA or diclofenac, respectively. Levels of malondialdehyde (MDA) and glutathione (GSH) were measured in plasma samples. Enzyme activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured. The paws of rats were evaluated histopathologically to investigate the anti-inflammatory effects. TNF-alpha levels were measured for the evaluation of inflammation. In Group II plasma MDA increased, levels of glutathione decreased, enzyme activities of SOD and GPx decreased. Histopathological damage increased in the paws of the rats in this group. MDA levels decreased in Groups III-V when compared with Group II. GSH levels significantly increased in Group III and IV than Group V. SOD activity of Group IV was higher than Group III and V. TNF-alpha levels were significantly lower in Group IV and V. LC and alpha-LA seemed to have protective effects against oxidative damage in adjuvant arthritis model.
自由基在佐剂性关节炎的病理生理学中起重要作用。本研究的目的是评估已知具有抗氧化作用的左旋肉碱（LC）和α-硫辛酸（α-LA）在治疗佐剂性关节炎中的疗效。通过给40只雄性Sprague-Dawley大鼠中的32只注射完全弗氏佐剂（CFA）建立关节炎模型。将大鼠分为五组。第一组大鼠作为对照，接受0.1 ml kg(-1)生理盐水。第二组仅接受0.1 ml CFA，作为其他组的CFA对照。第三至五组在注射CFA后，分别用LC、α-LA或双氯芬酸治疗。测量血浆样本中丙二醛（MDA）和谷胱甘肽（GSH）的水平。测量超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的酶活性。对大鼠的爪子进行组织病理学评估以研究抗炎作用。测量TNF-α水平以评估炎症。在第二组中，血浆MDA增加，谷胱甘肽水平降低，SOD和GPx的酶活性降低。该组大鼠爪子的组织病理学损伤增加。与第二组相比，第三至五组的MDA水平降低。第三组和第四组的GSH水平显著高于第五组。第四组的SOD活性高于第三组和第五组。第四组和第五组的TNF-α水平显著较低。LC和α-LA似乎对佐剂性关节炎模型中的氧化损伤具有保护作用。
