Shim Jae-Yoon, Jun Jong Kwan, Jung Bok-Kyung, Kim Sung Hoon, Won Hye-Sung, Lee Pil Ryang, Kim Ahm
Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Am J Obstet Gynecol. 2007 Sep;197(3):271.e1-4. doi: 10.1016/j.ajog.2007.06.045.
The purpose of this study was to evaluate potential associations between vascular endothelial growth factor (VEGF) gene polymorphisms and preeclampsia.
One hundred ten patients with preeclampsia and 209 healthy pregnant control subjects were enrolled in the study. After peripheral blood was obtained from all women and the genomic DNA was isolated, we genotyped +936C/T polymorphisms in the 3'-untranslated region of the VEGF gene, using polymerase chain reaction and restriction fragment length polymorphism techniques.
The distribution of genotypes of the +936C/T polymorphism was significantly different between women with preeclampsia and the control group (P < .001). Carriage of the +936T allele was significantly more frequent in preeclamptic patients than in control subjects (odds ratio, 2.06; 95% CI,1.38-3.08). Logistic regression analysis on VEGF genotype and clinical parameters such as age, educational status, body mass index, and neonatal gender showed carriage of the 936T allele to be significantly more frequent in preeclamptic patients than in control subjects (adjusted odds ratio, 2.23; 95% CI, 1.46-3.42).
Carriage of the +936T allele of the VEGF gene may be associated with increased susceptibility to the development of preeclampsia and may be an independent risk factor for preeclampsia.
本研究旨在评估血管内皮生长因子（VEGF）基因多态性与子痫前期之间的潜在关联。
110例子痫前期患者和209例健康孕妇作为对照纳入本研究。采集所有女性的外周血并分离基因组DNA后，采用聚合酶链反应和限制性片段长度多态性技术对VEGF基因3'-非翻译区的+936C/T多态性进行基因分型。
子痫前期患者与对照组之间+936C/T多态性的基因型分布存在显著差异（P <.001）。子痫前期患者携带+936T等位基因的频率显著高于对照组（优势比，2.06；95%可信区间，1.38 - 3.08）。对VEGF基因型与年龄、教育程度、体重指数和新生儿性别等临床参数进行逻辑回归分析显示，子痫前期患者携带936T等位基因的频率显著高于对照组（校正优势比，2.23；95%可信区间，1.46 - 3.42）。
VEGF基因+936T等位基因的携带可能与子痫前期发病易感性增加有关，可能是子痫前期的一个独立危险因素。
